https://scholar.dgist.ac.kr/handle/20.500.11750/882 2026-04-04T16:38:03Z https://scholar.dgist.ac.kr/handle/20.500.11750/58809 Title: 新規多能性細胞 Author(s): 손미영; 하정민; 정경숙; 남주현; 이정수; 김종경; 전영주; 백아름; 김장환 Abstract: 本発明は、非多能性細胞を多能性細胞に変換する方法；該方法により産生された細胞；およびその使用に関する。本発明の多能性細 https://scholar.dgist.ac.kr/handle/20.500.11750/58706 Title: NOVEL PLURIPOTENT CELLS Author(s): 손미영; 남주현; 김종경; 하정민; 김장환; 정경숙; 이정수; 백아름; 전영주 Abstract: The present invention relates to: a method for converting non-pluripotent cells into pluripotent cells; cells produced by the method; and uses thereof. The pluripotent cells of the present invention have low tumorigenicity while having excellent cell differentiation ability and pluripotency capable of differentiating into three germ layers, and can specifically separate pluripotent cells in the intermediate stage of reprogramming, and thus, it is possible to produce pluripotent cells having characteristics different from conventional induced pluripotent stem cells. https://scholar.dgist.ac.kr/handle/20.500.11750/57447 Title: Gut Microbiota Defines Functional Direction of Colonic Regulatory T Cells with Unique TCR Repertoires Author(s): Byun, Seohyun; Lee, Jusung; Choi, Yoon Ha; Ko, Haeun; Lee, Changhon; Park, John Chulhoon; Kim, Seung Won; Lee, Haena; Sharma, Amit; Kim, Kwang Soon; Rudra, Dipayan; Kim, Jong Kyoung; Im, Sin-Hyeog Abstract: Intestinal microbiota and selected strains of commensal bacteria influence regulatory T (Treg) cell functionality in the colon. Nevertheless, whether and how microbiota changes the transcriptome profile and TCR specificities of colonic Tregs remain to be precisely defined. In this study, we have employed single-cell RNA sequencing and comparatively analyzed colonic Tregs from specific pathogen-free and germ-free (GF) mice. We found that microbiota shifts the activation trajectory of colonic Tregs toward a distinct phenotypic subset enriched in specific pathogen-free but not in GF mice. Moreover, microbiota induced the expansion of specific Treg clonotypes with shared transcriptional specificities. The microbiota-induced subset of colonic Tregs, identified as PD-12 CXCR3+ Tregs, displayed enhanced suppressive capabilities compared with colonic Tregs derived from GF mice, enhanced production of IL-10, and were the primary regulators of enteric inflammation in dextran sodium sulfate-induced colitis. These findings identify a hitherto unknown gut microbiota and immune cell interaction module that could contribute to the development of a therapeutic modality for intestinal inflammatory diseases. © 2024 by The American Association of Immunologists, Inc. 2024-08-31T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/57410 Title: Single-cell transcriptomic analysis reveals dynamic activation of cellular signaling pathways regulating beige adipogenesis Author(s): Kyung, Dong Soo; Lee, Eun Min; Chae, Sehyun; Son, Yeonho; Moon, Ye-Jin; Hwang, Daehee; Kim, Jong Kyoung; Lee, Yun-Hee; Seong, Je Kyung Abstract: PDGFRA+ cells have been identified as adipocyte stem cells (ASCs) that differentiate into beige adipocytes in white adipose tissue (WAT) following thermogenic stimuli. To elucidate the molecular heterogeneity of ASCs, we conducted single-cell transcriptomic profiling of PDGFRA+ cells isolated from the inguinal WAT (iWAT) of mice treated with the beta3 adrenergic receptor agonist CL316243. Single-cell RNA-seq revealed nine major clusters, which were categorized into four groups: resting, proliferating, differentiating, and adipogenic factor-expressing cells (AFECs). Trajectory analysis revealed sequential activation of molecular pathways, including the Hedgehog and Notch signaling pathways, during beige adipogenesis. AFECs expressed Dpp4 and did not differentiate into adipocytes in culture or after transplantation. Furthermore, genetic lineage tracing studies indicated that DPP4+ cells did not differentiate into adipocytes in iWAT during CL316243-induced beige adipogenesis. However, high-fat diet feeding led to the recruitment of adipocytes from DPP4+ cells in iWAT. Overall, this study improved our understanding of the dynamic molecular basis of beige adipogenesis and the potential contribution of DPP4+ adipocyte lineages to the pathological expansion of WAT during diet-induced obesity. © The Author(s) 2024. 2024-09-30T15:00:00Z