https://scholar.dgist.ac.kr/handle/20.500.11750/9983 2026-04-22T09:43:25Z https://scholar.dgist.ac.kr/handle/20.500.11750/59140 Title: The chloroplast-targeted long noncoding RNA CHLORELLA mediates chloroplast functional transition across leaf ageing via anterograde signalling Author(s): Kang, Myeong Hoon; Lee, Juhyeon; Kim, Jinkwang; Mohammad, Hazara Begum; Park, Jeehye; Jung, Hyun Ju; Kim, Seonghwan; Lee, Heeho; Yang, Seong Wook; Kwak, June Myoung; Kim, Min-Sik; Lee, Jong-Chan; Lim, Pyung Ok Abstract: The transition from chloroplast biogenesis to degeneration during leaf senescence is critical for plants’ fitness, as it facilitates the relocation of nutrients to reproductive organs1, 2–3. However, it remains largely unknown how the timing of this transition is regulated by the coordination between chloroplasts and the nucleus4,5. Here we describe the regulatory mechanism underlying this transition in Arabidopsis thaliana. CHLOROPLAST-RELATED LONG NONCODING RNA (CHLORELLA) is highly co-expressed with genes supporting chloroplast function during leaf development. Leaves lacking CHLORELLA exhibit precocious senescence and reduced expression of chloroplast-associated genes, suggesting that CHLORELLA helps maintain chloroplast function. Mechanistically, CHLORELLA transcripts are translocated into chloroplasts and contribute to the accumulation of the plastid-encoded RNA polymerase complex. As leaves age, the expression of CHLORELLA decreases, leading to reduced plastid-encoded RNA polymerase accumulation and diminished transcription of photosynthesis-related genes, which may trigger leaf senescence. Moreover, CHLORELLA expression is activated by GOLDEN2-LIKE1 and GOLDEN2-LIKE2, master regulators of chloroplast development6, 7–8. Our study unravels a long-noncoding-RNA-based anterograde signalling mechanism that facilitates timely leaf senescence. © 2025 Elsevier B.V., All rights reserved. 2025-10-31T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/59138 Title: STED 현미경 이미지 처리 방법 및 STED 현미경 시스템 Author(s): 이종찬; 정세주; 김재용; 고동빈 Abstract: 본 발명은 STED 현미경 이미지 처리 방법 및 STED 현미경 시스템을 제공하며, 측정 대상에 여기 레이저 빔 및 제1 STED 레이저 빔을 조사하여 얻은 제1 메인 이미지와, 상기 측정 대상에 상기 제1 STED 레이저 빔만을 조사하여 얻은 제1 보조 이미지를 이용하여 제1 STED 이미지를 획득하는 단계, 상기 측정 대상에 상기 여기 레이저 빔 및 상기 제1 STED 레이저 빔과 세기가 다른 제2 STED 레이저 빔을 조사하여 얻은 제2 메인 이미지와, 상기 측정 대상에 제2 STED 레이저 빔만을 조사하여 얻은 제2 보조 이미지를 이용하여 제2 STED 이미지를 획득하는 단계 및 상기 제1 STED 이미지 및 상기 제2 STED 이미지를 이용하여 배경잡음을 제거한 최종 이미지를 획득하는 단계를 포함할 수 있다. https://scholar.dgist.ac.kr/handle/20.500.11750/58737 Title: STED MICROSCOPE IMAGE PROCESSING METHOD AND STED MICROSCOPE SYSTEM Author(s): 정세주; 김재용; 고동빈; 이종찬 Abstract: The present invention provides a STED microscope image processing method and a STED microscope system, the method comprising the steps of: obtaining a first STED image by using a first main image, which is obtained by emitting an excitation laser beam and a first STED laser beam at an object being measured, and a first auxiliary image, which is obtained by emitting only the first STED laser beam at the object being measured; obtaining a second STED image by using a second main image, which is obtained by emitting, at the object being measured, the excitation laser beam and a second STED laser beam of an intensity different from that of the first STED laser beam, and a second auxiliary image, which is obtained by emitting only the second STED laser beam at the object being measured; and using the first STED image and the second STED image so as to obtain a final image from which background noise is removed. https://scholar.dgist.ac.kr/handle/20.500.11750/58662 Title: High-temperature-induced FKF1 accumulation promotes flowering through the dispersion of GI and degradation of SVP Author(s): Lee, Hong Gil; Kim, Jinkwang; Park, Kyung-Ho; Lee, Hongwoo; Kim, Sol-Bi; Jung, Ji-Yul; Gwak, Eunha; Ahn, Ji Hoon; Jung, Jae-Hoon; Lee, Jong-Chan; Seo, Pil Joon Abstract: Floral transition is influenced by photoperiod and ambient temperature, which are integrated to modulate development via a molecular mechanism that remains to be elucidated. Here we demonstrate that the F-box protein FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 (FKF1) and its interacting partner GIGANTEA (GI), central regulators of photoperiodic flowering, target SHORT VEGETATIVE PHASE (SVP) for 26S-proteasome-dependent degradation to regulate the temperature-responsive developmental transition to flowering. At low temperatures, GI is sequestered in liquid-like nuclear condensates. By contrast, FKF1 accumulates at high temperatures and releases GI from condensates to form a nuclear-dispersed FKF1–GI complex, leading to SVP degradation under short-day conditions. Temperature sensitivity is significantly reduced in fkf1-t, gi-2 and fkf1-2 gi-2 mutants. We propose that the FKF1–GI complex mediates the proteolysis of a floral repressor via reversible liquid–liquid phase separation to accelerate floral transition at high temperatures. © The Author(s), under exclusive licence to Springer Nature Limited 2025. 2025-06-30T15:00:00Z