https://scholar.dgist.ac.kr/handle/20.500.11750/9983 2026-09-13T08:24:55Z https://scholar.dgist.ac.kr/handle/20.500.11750/60685 Title: Arabidopsis WSS1A, a DNA-protein crosslink repair protease, delays leaf senescence in cooperation with SUMO3 Author(s): Park, Sanghoon; Oh, Hyunwoo; Jeong, Ukcheol; Lee, Jae Ho; Choi, Hyeyoung; Park, Hyunsu; Kim, Jinkwang; Kim, Yongmin; Kwak, Junmin; Yoon, Yeong Seon; Li, Zhonghai; Lee, Jong-Chan; Woo, Hye Ryun Abstract: Leaf senescence, the final stage of leaf development, is regulated by complex interplays of intrinsic genetic programs and environmental cues. Throughout their lifetimes, all living organisms encounter various endogenous and environmental challenges, many of which can cause potentially fatal DNA damage. Among these, DNA-protein crosslinks (DPCs) are particularly deleterious, as they obstruct essential processes such as replication and transcription, thereby compromising genome integrity and ultimately leading to premature aging across species. However, the biological significance of DPCs and their repair mechanisms in leaf senescence remains unexplored. Here, we demonstrate that cis-platin (cis-Pt), a potent DPC inducer, accelerates leaf senescence in Arabidopsis. We reveal that Arabidopsis DPC repair factor WSS1A, a WLM/Spr-T metalloprotease, plays a negative role in leaf senescence induced by cis-Pt treatment, darkness, and leaf age. WSS1A forms nuclear condensates via liquid-liquid phase separation both in vitro and in vivo, which is cooperatively driven by its N-terminal segment and intrinsically disordered region. Mechanistically, WSS1A non-covalently interacts with SMALL UBIQUITIN MODIFIER 3 (SUMO3) through its SUMO-interacting motif and is also covalently SUMOylated by SUMO3. Genetic analysis further reveals that WSS1A and SUMO3 act in the same pathway to control cis-Pt-induced leaf senescence. Together, this study establishes a conceptual framework connecting DPC repair and SUMO3-dependent regulation of WSS1A in modulating leaf senescence in Arabidopsis. 2026-07-31T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/59140 Title: The chloroplast-targeted long noncoding RNA CHLORELLA mediates chloroplast functional transition across leaf ageing via anterograde signalling Author(s): Kang, Myeong Hoon; Lee, Juhyeon; Kim, Jinkwang; Mohammad, Hazara Begum; Park, Jeehye; Jung, Hyun Ju; Kim, Seonghwan; Lee, Heeho; Yang, Seong Wook; Kwak, June Myoung; Kim, Min-Sik; Lee, Jong-Chan; Lim, Pyung Ok Abstract: The transition from chloroplast biogenesis to degeneration during leaf senescence is critical for plants’ fitness, as it facilitates the relocation of nutrients to reproductive organs1, 2–3. However, it remains largely unknown how the timing of this transition is regulated by the coordination between chloroplasts and the nucleus4,5. Here we describe the regulatory mechanism underlying this transition in Arabidopsis thaliana. CHLOROPLAST-RELATED LONG NONCODING RNA (CHLORELLA) is highly co-expressed with genes supporting chloroplast function during leaf development. Leaves lacking CHLORELLA exhibit precocious senescence and reduced expression of chloroplast-associated genes, suggesting that CHLORELLA helps maintain chloroplast function. Mechanistically, CHLORELLA transcripts are translocated into chloroplasts and contribute to the accumulation of the plastid-encoded RNA polymerase complex. As leaves age, the expression of CHLORELLA decreases, leading to reduced plastid-encoded RNA polymerase accumulation and diminished transcription of photosynthesis-related genes, which may trigger leaf senescence. Moreover, CHLORELLA expression is activated by GOLDEN2-LIKE1 and GOLDEN2-LIKE2, master regulators of chloroplast development6, 7–8. Our study unravels a long-noncoding-RNA-based anterograde signalling mechanism that facilitates timely leaf senescence. © 2025 Elsevier B.V., All rights reserved. 2025-10-31T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/59138 Title: STED 현미경 이미지 처리 방법 및 STED 현미경 시스템 Author(s): 이종찬; 정세주; 김재용; 고동빈 Abstract: 본 발명은 STED 현미경 이미지 처리 방법 및 STED 현미경 시스템을 제공하며, 측정 대상에 여기 레이저 빔 및 제1 STED 레이저 빔을 조사하여 얻은 제1 메인 이미지와, 상기 측정 대상에 상기 제1 STED 레이저 빔만을 조사하여 얻은 제1 보조 이미지를 이용하여 제1 STED 이미지를 획득하는 단계, 상기 측정 대상에 상기 여기 레이저 빔 및 상기 제1 STED 레이저 빔과 세기가 다른 제2 STED 레이저 빔을 조사하여 얻은 제2 메인 이미지와, 상기 측정 대상에 제2 STED 레이저 빔만을 조사하여 얻은 제2 보조 이미지를 이용하여 제2 STED 이미지를 획득하는 단계 및 상기 제1 STED 이미지 및 상기 제2 STED 이미지를 이용하여 배경잡음을 제거한 최종 이미지를 획득하는 단계를 포함할 수 있다. https://scholar.dgist.ac.kr/handle/20.500.11750/58737 Title: STED MICROSCOPE IMAGE PROCESSING METHOD AND STED MICROSCOPE SYSTEM Author(s): 정세주; 김재용; 고동빈; 이종찬 Abstract: The present invention provides a STED microscope image processing method and a STED microscope system, the method comprising the steps of: obtaining a first STED image by using a first main image, which is obtained by emitting an excitation laser beam and a first STED laser beam at an object being measured, and a first auxiliary image, which is obtained by emitting only the first STED laser beam at the object being measured; obtaining a second STED image by using a second main image, which is obtained by emitting, at the object being measured, the excitation laser beam and a second STED laser beam of an intensity different from that of the first STED laser beam, and a second auxiliary image, which is obtained by emitting only the second STED laser beam at the object being measured; and using the first STED image and the second STED image so as to obtain a final image from which background noise is removed.