https://scholar.dgist.ac.kr/handle/20.500.11750/1158 Sat, 04 Apr 2026 16:37:37 GMT 2026-04-04T16:37:37Z https://scholar.dgist.ac.kr/handle/20.500.11750/58672 Title: Mag-centrifugal microfluidic separation for rapid purification of dorsal root ganglion neurons Author(s): Lee, Hee Jae; Kim, Ji Hoon; Kim, Woon-Hae; Shin, Hyun Young; Lee, Seung Joon; Sunoo, Joseph; Mo, Yun Jeong; Kim, Yu Seon; Lee, Yun Il; Kim, Minseok S. Abstract: Dorsal root ganglion (DRG) neurons have been widely used as a good model for sensory neurons of the peripheral nervous system in neurology. However, purification process is essential to only acquire the sensory neurons since the DRG organ is composed of several types of cells including fibroblast, Schwann and epithelial cells. In this study, we introduce a novel hybrid isolation method combining centrifugal and immunomagnetic separations to selectively remove the Schwann cells, fibroblasts and epithelial cells with fast manner. As a result, we obtained high purity and high yield of primary sensory neurons and achieved a reliable and rapid purification method through an automated process. Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved. Sat, 10 Nov 2018 15:00:00 GMT https://scholar.dgist.ac.kr/handle/20.500.11750/58672 2018-11-10T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/46975 Title: A high throughput screening platform to rejuvenate skeletal muscle function via electrical stimulation Author(s): Kim, Min Young; Shin, Hyun Young; Lee, Seung Joon; Kim, Minseok S. Abstract: Sarcopenia is the disease owing to loss of muscle tissue as a natural part of the aging process and it has not yet developed drugs. Unfortunately, although exercise is the only way to treat sarcopenia, it is sometimes difficult for elderly people to exercise by various inconvenient body conditions. While many studies have been done to apply electrical stimulation (ES) as an alternative intervention of exercise, there are still unmet demands to investigate the effect of precise and systemic ES for skeletal muscle cells (skMC) as well as to explore the behavior of aged skMC. As such, we developed a novel ES-based high throughput screening platform to quantitatively search the ES effect for skMC. Using this platform, we could see the possibility to improve the functions of old skMC cells via ES. © 2019 CBMS-0001. Mon, 28 Oct 2019 15:00:00 GMT https://scholar.dgist.ac.kr/handle/20.500.11750/46975 2019-10-28T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/46962 Title: Epigenetic regulation of epithelial-to-mesenchymal transition as a captain of journey of cancer from lung to brain Author(s): Lee, YM; Kim, SH; Kim, Minseok S.; Kim, YZ Abstract: Background: There have been many studies for epithelial-to-mesenchymal transition (EMT) of the cancer, especially focused on the important transcriptional factors. Recently epigenetic mechanisms, including histone modification have been comprehensively studied for establishing the role on the prohibiting metastasis of cancer cell. The objective of presenting study is to investigate the epigenetic role of histone lysine methylation/demethylation on the regulating expression of the transcriptional factors of EMT in lung cancer and brain metastasis. Material and Methods: The paired samples of NSCLC and brain metastasis were analyzed in 23 individual patients. Both samples were obtained by surgical resection or biopsy of the lung and brain. The paraffin-fixed formalin embedded (FFPE) samples was obtained at the pathological archives in our institute. The medical record (age, gender, performance status, interval of metastasis, site of extracranial metastasis, survival after diagnosis of NSCLC, and survival after diagnosis of brain metastasis) was reviewed retrospectively. In both samples of NSCLC and brain metastasis, immunohistochemical staining was performed for epithelial markers, mesenchymal markers, transcriptional factors of EMT factors, histone lysine methyltransferase, and histone lysine demethylase. Results: The epithelial markers such as E-cadherin (24.6% vs 12.6%, p = 0.037), Desmoplakin (15.6% vs 2.3%, p = 0.007), α-caterin (41.3% vs 28.3%, p = 0.042), and β-caterin (38.6% vs 16.9%, p = 0.029) were significantly decreased in brain metastasis samples compared with NSCLC sample. The mesenchymal markers such as N-cadherin (20.6% vs 43.2%, p = 0.028), Vimentin (15.3% vs 51.6%, p = 0.004), and Fibronectin (7.6% vs 39.4%, p = 0.002) were significantly increased in brain metastasis samples compared with NSCLC sample. The immunoreactivity of transcriptional factors such as Slug (15.6% vs 42.6%, p = 0.005), Twist (23.6% vs 45.9%, p = 0.010) and ZEB1 (15.0% vs 55.9%, p = 0.002) was also increased in brain metastasis samples compared with NSCLC sample. Epigenetic inducers such as MLL4 (H3K4 methyltransferase) and UTX (H3K36 demethylase) were statistically increased and epigenetic repressor such as EZH2 (H3K27 methyltransferase) was statistically decreased in brain metastasis samples compared with NSCLC sample. The expression of UTX-ZEB1 (R2 linear = 1.204) and MLL4-Slug (R2 linear = 0.987) was increased in direct proportion and EZH2-Twist (R2 linear = − 2.723) was decreased in reverse proportion. Conclusion. This study suggested that MLL4 and UTX should epigenetically induce the expression of EMT transcriptional factor such as Slug and ZEB1 respectively, and EZH2 should epigenetically reduce the expression of EMT transcription factor such as Twist in the process of NSCLC metastasis to the brain. Sat, 24 Oct 2020 15:00:00 GMT https://scholar.dgist.ac.kr/handle/20.500.11750/46962 2020-10-24T15:00:00Z