https://scholar.dgist.ac.kr/handle/20.500.11750/528 Sat, 04 Apr 2026 19:03:41 GMT 2026-04-04T19:03:41Z https://scholar.dgist.ac.kr/handle/20.500.11750/58903 Title: Intestinal therapeutic agent delivery microrobot with magnetic targeting and pH-triggered degradation abilities Author(s): Lee, Hyoryong; Mun, Na Eun; Yoon, Deockhee; Choi, Yun-Jung; Park, Jam-Eon; Kim, Dong-Jae; Yoo, Su Woong; Park, Seung-Hwan; Park, Sukho Abstract: Oral drug delivery remains a clinically preferred route for colorectal cancer therapy due to its noninvasive nature and patient compliance. However, conventional formulations suffer from premature degradation in the gastric environment and poor site-specific targeting in the intestine, significantly reducing therapeutic efficacy. Here, we introduce an intestinal therapeutic agent delivery microrobot (ITAM) composed of a therapeutic agent (TA) encapsulated core, a magnetic nanoparticle (MNP) layer for precise magnetic guidance, and a protective layer to minimize the loss of functional materials. This layered structure enables gastric-resilient transit, magnetic localization to colorectal lesions, and colonic pH-triggered release of TAs. In vivo studies demonstrate that ITAM achieves superior lesion-specific targeting and therapeutic efficacy enhancement compared to conventional oral delivery. Furthermore, ITAM serves as a versatile platform for delivering various therapeutic substances, including drugs, cells, and beneficial microorganisms, broadening its potential applications in intestinal disease treatment and gut health modulation. Sat, 31 Jan 2026 15:00:00 GMT https://scholar.dgist.ac.kr/handle/20.500.11750/58903 2026-01-31T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/58687 Title: Comprehensive Osteosarcoma Treatment with Multifunctional Composite Hydrogels Enabling Combined Photothermal Cancer Ablation and Osteoinductive Tissue Regeneration Author(s): Byun, Hayeon; Hwang, Taeyeon; Lee, Hyoryong; Choi, Yun-Jung; Kim, Dong-Jae; Park, Eunji; Kim, Eunhyung; Park, Sukho; Shin, Heungsoo Abstract: Osteosarcoma treatment can lead to considerable loss of bone tissue, creating a challenging microenvironment for recovery. Here, a novel biomaterial is described for tumor treatment via photothermal therapy and bone-tissue regeneration. Multifunctional composite hydrogels can be fabricated by incorporating mineralized magnetic fibers (G-mMFs) into a gelatin-genipin hydrogel. The G-mMFs exhibit notable temperature increases in response to near-infrared irradiation, and superior disruption of tumor tissue follows hyperthermia therapy in a tumor-bearing mouse model. G-mMFs protect stem cells from the oxidative stress anticipated after tumor ablation, following significant increases in catalase and anti-apoptotic gene expression. G-mMFs demonstrate enhanced osteoinductivity, with nearly 90% of human adipose-derived stem cells exhibiting osteogenic markers. Adenosine signaling-mediated osteogenesis and restoration of osteogenesis under oxidative stress can be demonstrated through stem-cell differentiation in the presence of H2O2. In vivo, regeneration of bone tissue can be assessed using a calvarial bone-defect mouse model, with nearly twice the amount of bone formation in the G-mMF group compared with mice without implantation, along with a more mature bone-tissue structure. Collectively, these study results present G-mMFs as a multifunctional biomaterial that simultaneously addresses tumor ablation and bone regeneration, offering a promising strategy for the comprehensive treatment of osteosarcoma. Wed, 31 Dec 2025 15:00:00 GMT https://scholar.dgist.ac.kr/handle/20.500.11750/58687 2025-12-31T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/57299 Title: Astrocytic inhibition of lateral septal neurons promotes diverse stress responses Author(s): Seo, Kain; Won, Sanghyun; Lee, Hee-Yoon; Sin, Yeonju; Lee, Sangho; Park, Hyejin; Kim, Yong Geon; Yang, Seo Young; Kim, Dong-Jae; Suk, Kyoungho; Koo, Ja Wook; Baek, Myungin; Choi, Se-Young; Lee, Hyosang Abstract: Inhibitory neuronal circuits within the lateral septum (LS) play a key role in regulating mood and stress responses. Even though glial cells can modulate these circuits, the impact of astrocytes on LS neural circuits and their functional interactions remains largely unexplored. Here, we demonstrate that astrocytes exhibit increased intracellular Ca²⁺ levels in response to aversive sensory and social stimuli in both male and female mice. This astrocytic Ca²⁺ elevation inhibits neighboring LS neurons by reducing excitatory synaptic transmissions through A1R-mediated signaling in both the dorsal (LSd) and intermediate LS (LSi) and enhancing inhibitory synaptic transmission via A2AR-mediated signaling in the LSi. At the same time, astrocytes reduce inhibitory tone on distant LS neurons. In the LSd, astrocytes promote social avoidance and anxiety, as well as increased heart rate in socially stressed male mice. In contrast, astrocytes in the LSi contribute to elevated heart rate and heightened blood corticosterone levels in unstressed male mice. These results suggest that the dynamic interactions between astrocytes and neurons within the LS modulate physiological and behavioral responses to stressful experiences. © The Author(s) 2024. Thu, 31 Oct 2024 15:00:00 GMT https://scholar.dgist.ac.kr/handle/20.500.11750/57299 2024-10-31T15:00:00Z https://scholar.dgist.ac.kr/handle/20.500.11750/47953 Title: Effect of sp(3)/sp(2) carbon ratio and hydrodynamic size on the biodistribution kinetics of nanodiamonds in mice via intravenous injection Author(s): Jeong, Jiyoung; Jeon, Soyeon; Kim, Songyeon; Lee, Sinuk; Kim, Gyuri; Bae, Eunsol; Ha, Yeonjeong; Lee, Seung Whan; Kim, Ji-Su; Kim, Dong-Jae; Cho, Wan-Seob Abstract: Background: Nanodiamonds (NDs) have gained a rapidly growing interest in biomedical applications; however, little is known regarding their biokinetics owing to difficulties in measurements and limited synthesis/purification technologies. In this study, we investigated the distribution kinetics of detonation-synthesized NDs in mice via intravenous injection to evaluate the parameters that determine the behavior of the particles. We prepared two distinctive NDs that controlled the sp3 /sp2 carbon ratio and particle size by coating them with serum proteins. The four control samples were intravenously injected into mice, and tissue distribution and clearance were evaluated at 30 min and 1, 7, and 28 days post-injection. Results: The sp3 /sp2 carbon ratio showed no correlation with the organ distribution of the NDs. However, hydrodynamic size showed an excellent correlation with organ distribution levels: a negative correlation in the liver and positive correlations in the spleen and lungs. Furthermore, the deposition levels of NDs in the lung suggest that particles smaller than 300 nm could avoid lung deposition. Finally, a similar organ distribution pattern was observed in mice injected with carbon black nanoparticles controlled hydrodynamic size. Conclusions: In conclusion, the tissue distribution of NDs is modulated not by the sp3 /sp2 carbon ratio but by the hydrodynamic size, which can provide helpful information for targeting the tissue of NDs. Furthermore, the organ distribution pattern of the NDs may not be specific to NDs but also can apply to other nanoparticles, such as carbon black. © 2023, BioMed Central Ltd., part of Springer Nature. Mon, 31 Jul 2023 15:00:00 GMT https://scholar.dgist.ac.kr/handle/20.500.11750/47953 2023-07-31T15:00:00Z