Cited 0 time in webofscience Cited 0 time in scopus

Removal of leukemia cells in the circulating system by magnetic hyperthermia and the prediction of temperature changes on the cell surface and blood stream

Title
Removal of leukemia cells in the circulating system by magnetic hyperthermia and the prediction of temperature changes on the cell surface and blood stream
Authors
Faruque, Md Hasan AlChoi, Eun-SookLee, HyoryongKim, Jung-HeePark, SukhoKim, Eunjoo
DGIST Authors
Kim, Jung-Hee; Park, SukhoKim, Eunjoo
Issue Date
2019-11-01
Citation
2019 KSIEC fall Meeting 2019 한국공업화학회 추계 총회 및 학술대회
Type
Conference
Abstract
Removal of leukemia cells in the circulating system by magnetic hyperthermia and the prediction of temperature changes on the cell surface and blood stream Hasan Al Faruque,1 Eun-Sook Choi,1 Hyo-Ryong Lee,2 Jung-Hee Kim,1 Seok-Ho Park,2 Eunjoo Kim1 Companion Diagnostics and Medical Technology Research Group, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, Republic of Korea, 42988 Department of Robotics Engineering, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, Republic of Korea, 42988 Leukemia cells in the bloodstream were directly removed under whole-body hyperthermia, using leukemia cell-specific MNPs. EpCAM antibody was immobilized on the surface of MNPs (EpCAM-MNPs) to introduce the specificity of MNPs to leukemia cells. The efficiency of EpCAM-MNPs for removal of THP1 cells was assessed under magnetic hyperthermia in vitro. In AKR mice, an animal model of lymphoblastic leukemia, the number of leukemia cells was measured following the intravenous injection of EpCAM-MNPs and subsequent whole-body hyperthermia treatment. To support the results, simulation analysis of heat transfer from MNPs to leukemia cells was performed using COMSOL Multiphysics simulation software. The proliferation of THP1 cells was decreased to 40.8% of that in control samples by hyperthermia with EpCAM-MNPs. After the whole-body hyperthermia to AKR mice following the injection of EpCAM-MNPs, the leukemia cell number was also decreased to 43.8% of that without the treatment of hyperthermia, determined by Leishman staining of leukemia cells. The simulation of heat generation by MNPs showed that the surface temperature of leukemia cells adhered to EpCAM-MNPs could be increased to 82C. However, the temperature of free cells without adhered MNPs was predicted as 38C. Magnetic hyperthermia can be used to remove leukemia cells owing to the specific binding of MNPs to target cells. This approach has potential as a therapeutic to remove metastatic cancer cells, and pathogenic bacteria and viruses floating in the bloodstream.
URI
http://hdl.handle.net/20.500.11750/14201
Publisher
한국공업화학회
Related Researcher
  • Author Kim, Eunjoo  
  • Research Interests Biomarker, liquid biopsy, molecular diagnosis, nanobiosensor, drug delivery, exosome
Files:
There are no files associated with this item.
Collection:
Division of Electronics & Information System2. Conference Papers
Department of Robotics and Mechatronics EngineeringMultiscale Biomedical Robotics Laboratory2. Conference Papers


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE