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Graphene layer substrate assisted laser desorption at atmospheric pressure for high spatial resolution mass spectrometry imaging

Graphene layer substrate assisted laser desorption at atmospheric pressure for high spatial resolution mass spectrometry imaging
Kim, Jae YoungLee, SunYoungLim, Hee JinPark, Ji-WonLim, Dong-KwonMoon, DaeWon
DGIST Authors
Kim, Jae Young
Issue Date
22nd International Mass Spectrometry Conference 2018
Introduction: We are demonstrating a new method that can facilitate the laser desorption process without gold nanoparticle treatment step. The use of graphene layer substrate is excellent platform to induce the desorption process using 532 nm-continuous wave (CW) laser. By using this method, high spatial resolution atmospheric pressure mass spectrometry (AP-MS) imaging of humid tissues can be obtained. Methods: We used the previously developed AP-nanoPALDI MS system with a change in laser source [1]. One of the most widely used lasers, a 532nm CW laser, was used as the desorption source. A graphene layer substrate was prepared by transferring commercial CVD graphene on Cu foil to slide glass. The specimen was placed on graphene layer substrate and positioned on the scanning stage for MS imaging.Results: Many previous studies reported using graphene layer as a substrate for absorbing laser energy have shown that the laser source was located above the specimen and the laser light first met the specimen rather than the graphene layer [2-4]. Thus, in order for the graphene layer to role as a light absorbing layer, the thickness of the specimen had to be thin. In our proposed AP-MS system, since the laser light was focused on the specimen by the objective lens of the inverted type optical microscope, the laser source was placed under the specimen, so that the laser light was irradiated to the graphene layer first and the light energy was converted to heat from graphene layer and transferred to the tissue specimen. Therefore, micrometer-resolution ion images for mouse hippocampal tissue slices were obtained under ambient conditions.Conclusions: Compared to the preparation of the nanoparticles treated biological specimen, while the nanoparticle treatment of the specimen was omitted, the process of transferring the graphene layer was added to the slide glass substrate. The graphene layer substrates can be prepared and stored in advance, resulting in a simplified specimen preparation and a great advantage in preparing living tissue faster. Novel Aspect: The efficient laser desorption of bio-molecules on the wet-state hippocampal tissue is possible by use of graphene layer substrate.
Italian Chemical Society
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Department of Robotics and Mechatronics EngineeringRobotics Engineering Research Center2. Conference Papers

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