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Two guard cell mitogen-activated protein kinases, MPK9 and MPK12, function in methyl jasmonate-induced stomatal closure in Arabidopsis thaliana

Two guard cell mitogen-activated protein kinases, MPK9 and MPK12, function in methyl jasmonate-induced stomatal closure in Arabidopsis thaliana
Khokon, Md. A. R.Salam, M. A.Jammes, F.Ye, W.Hossain, M. A.Uraji, M.Nakamura, Y.Mori, I. C.Kwak, June MyoungMurata, Y.
Issued Date
Plant Biology, v.17, no.5, pp.946 - 952
Author Keywords
[Ca2+]cytoscillationscytosolic alkalisationguard cell, mitogen-activated protein kinasemethyl jasmonatereactive oxygen speciesS-type anion channels
Reactive Oxygen Species (ROS)RolesS Type Anion ChannelsSignal Transduction[Ca2+](Cyt) OscillationsABAOxygen Species ProductionPhosphatase PairAbscisic Acid (ABA)Anion Channel Slac1Cytosolic AlkalisationCytosolic CalciumGuard CellIon ChannelMap KinaseMethyl JasmonateMitogen Activated Protein Kinase
Methyl jasmonate (MeJA) and abscisic acid (ABA) signalling cascades share several signalling components in guard cells. We previously showed that two guard cell-preferential mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate ABA signalling in Arabidopsis thaliana. In this study, we examined whether these two MAP kinases function in MeJA signalling using genetic mutants for MPK9 and MPK12 combined with a pharmacological approach. MeJA induced stomatal closure in mpk9-1 and mpk12-1 single mutants as well as wild-type plants, but not in mpk9-1 mpk12-1 double mutants. Consistently, the MAPKK inhibitor PD98059 inhibited the MeJA-induced stomatal closure in wild-type plants. MeJA elicited reactive oxygen species (ROS) production and cytosolic alkalisation in guard cells of the mpk9-1, mpk12-1 and mpk9-1 mpk12-1 mutants, as well in wild-type plants. Furthermore, MeJA triggered elevation of cytosolic Ca2+ concentration ([Ca2+](cyt)) in the mpk9-1 mpk12-1 double mutant as well as wild-type plants. Activation of S-type anion channels by MeJA was impaired in mpk9-1 mpk12-1. Together, these results indicate that MPK9 and MPK12 function upstream of S-type anion channel activation and downstream of ROS production, cytosolic alkalisation and [Ca2+](cyt) elevation in guard cell MeJA signalling, suggesting that MPK9 and MPK12 are key regulators mediating both ABA and MeJA signalling in guard cells.
Related Researcher
  • 곽준명 Kwak, June Myoung 뉴바이올로지학과
  • Research Interests Calcium Signaling; ABA;ROS Signaling; plant development; Development of Abscission; Culluar Features; Signaling network; Environmental Regulation
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Department of New Biology Lab of Cell Signaling and Development 1. Journal Articles


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