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Presence of multiple peripheral circadian oscillators in the tissues controlling voiding function in mice

Title
Presence of multiple peripheral circadian oscillators in the tissues controlling voiding function in mice
Authors
노종윤한동희김미희고일규김성은박노헌조한경김계환김경진김창주조세형
Issue Date
2014-03
Citation
Experimental and Molecular Medicine, 46(3), 1-8
Type
Article
Article Type
Article
Keywords
bladdercircadian clocklumbar spinal cordperipheral oscillatorvoidingwater intake
ISSN
1226-3613
Abstract
Circadian clocks are the endogenous oscillators that harmonize a variety of physiological processes within the body. Although many urinary functions exhibit clear daily or circadian variation in diurnal humans and nocturnal rodents, the precise mechanisms of these variations are as yet unclear. In the present study, we demonstrate that Per2 promoter activity clearly oscillates in neonate and adult bladders cultured ex vivo from Per2::Luc knock-in mice. In subsequent experiments, we show that multiple local oscillators are operating in all the bladder tissues (detrusor, sphincter and urothelim) and the lumbar spinal cord (L4-5) but not in the pontine micturition center or the ventrolateral periaqueductal gray of the brain. Accordingly, the water intake and urine volume exhibited daily and circadian variations in young adult wild-type mice but not in Per1-/-Per2-/- mice, suggesting a functional clock-dependent nature of the micturition rhythm. Particularly in PDK mice, the water intake and urinary excretion displayed an arrhythmic pattern under constant darkness, and the amount of water consumed and excreted significantly increased compared with those of WT mice. These results suggest that local circadian clocks reside in three types of bladder tissue and the lumbar spinal cord and may have important roles in the circadian control of micturition function. © 2014 KSBMB. All rights reserved.
URI
http://hdl.handle.net/20.500.11750/5815
DOI
10.1038/emm.2013.153
Publisher
생화학분자생물학회
Files:
There are no files associated with this item.
Collection:
ETC1. Journal Articles


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