Cited 5 time in webofscience Cited 7 time in scopus

Analyses of Intravesicular Exosomal Proteins Using a Nano-Plasmonic System

Title
Analyses of Intravesicular Exosomal Proteins Using a Nano-Plasmonic System
Authors
Park, JongminIm, HyungsoonHong, SeonkiCastro, Cesar M.Weissleder, RalphLee, Hakho
DGIST Authors
Hong, Seonki
Issue Date
2018-02
Citation
ACS Photonics, 5(2), 487-494
Type
Article
Article Type
Article
Keywords
surface plasmon resonanceextracellular vesiclesexosomesnanohole arraysbiomarlcerscancer
ISSN
2330-4022
Abstract
Extracellular vesicles (EVs), including exosomes, are nanoscale membrane particles shed from cells and contain cellular proteins whose makeup could inform cancer diagnosis and treatment. Most analyses have focused on surface proteins while analysis of intravesicular proteins has been more challenging. Herein, we report an EV screening assay for both intravesicular and transmembrane proteins using a nanoplasmonic sensor. Termed iNPS (intravesicular nanoplasmonic system), this platform used nanohole-based surface plasmon resonance (SPR) for molecular detection. Specifically, we (i) established a unified assay protocol to detect intravesicular as well as transmembrane proteins; and (ii) engineered plasmonic substrates to enhance detection sensitivity. The resulting iNPS enabled sensitive (0.5 μL sample per marker) and high-throughput (a 10 × 10 array) detection for EV proteins. When applied to monitor EVs from drug-treated cancer cells, the iNPS assay revealed drug-dependent unique EV protein signatures. We envision that iNPS could be a powerful tool for comprehensive molecular screening of EVs. © 2017 American Chemical Society.
URI
http://hdl.handle.net/20.500.11750/6403
DOI
10.1021/acsphotonics.7b00992
Publisher
American Chemical Society
Related Researcher
  • Author Hong, Seonki Biomaterials & Biointerface Engineering Laboratory
  • Research Interests
Files:
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Collection:
Department of Emerging Materials ScienceBiomaterials & Biointerface Engineering Laboratory1. Journal Articles


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