Characterization of Synaptoporin in the Hippocampal Mossy Fiber Pathway
- Title
- Characterization of Synaptoporin in the Hippocampal Mossy Fiber Pathway
- Author(s)
- Kim, Sang Hee
- DGIST Authors
- Ko, Jaewon ; Kim, Sang Hee ; Lee, Kea Joo
- Advisor
- 고재원
- Co-Advisor(s)
- Lee, Kea Joo
- Issued Date
- 2019
- Awarded Date
- 2019-02
- Type
- Thesis
- Table Of Contents
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Ⅰ. Introduction 1
1.1 Synaptoporin expression in the mossy fiber pathway 1
1.2 Synaptic vesicle pools 2
1.3 Potential roles of synaptoporin in the mossy fiber pathway 3
Ⅱ. Material & Methods 5
2.1 Experimental animals 5
2.2 Primary hippocampal neuron culture 5
2.3 Immunocytochemistry (ICC) 6
2.4 SRRF (Super resolution radial fluctuations) imaging 6
2.5 Immunogold Electron Microscopy 7
2.6 TEM (Transmission electron microscopy) imaging 8
2.7 Viral vector / Stereotaxic virus injection 8
2.8 Immunohistochemistry (IHC) 9
2.9 CLSM (Confocal laser scanning microscopy) imaging 9
2.10 Image processing / Statistics 10
Ⅲ. Results 11
3.1 Synaptoporinlocalization at the synapse level (pre vs. post synaptic region) 11
3.2 Ultrastructural distribution of synaptoporin in the mossy fiber terminals 12
3.3 Synaptic remodeling by modulation of SPO levels 12
IⅤ. Discussion 19
4.1 Discussion 19
- URI
-
http://dgist.dcollection.net/common/orgView/200000171528
http://hdl.handle.net/20.500.11750/10696
- Degree
- MASTER
- Department
- Brain and Cognitive Sciences
- Publisher
- DGIST
- Related Researcher
-
-
Ko, Jaewon
- Research Interests Synapse Formation and Function; Neural Circuits; 뇌질환; animal model
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- Files in This Item:
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- Appears in Collections:
- Department of Brain Sciences Theses Master
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