Endocytosis of BDNF and its localization in the astrocyte
- Title
- Endocytosis of BDNF and its localization in the astrocyte
- Author(s)
- Sungryeong Yoon
- DGIST Authors
- Choe, Han Kyoung ; Yoon, Sungryeong ; Park, Hyungju
- Advisor
- 최한경
- Co-Advisor(s)
- Hyungju Park
- Issued Date
- 2019
- Awarded Date
- 2019-02
- Type
- Thesis
- Table Of Contents
-
I. Introduction 1
II. Materials and methods 3
2.1 Primary cell culture and transfection 3
2.2 Viral vectors for TrkB knockdown 3
2.3 Immunocytochemistry 3
2.4 Preparation of QD-BDNF conjugate 4
2.5 Preparation of extracellular solution for live-cell imaging 4
2.6 Live cell imaging 4
2.7 Data analysis 4
III. Results 6
3.1 QD-mBDNF as a tool for monitoring endosomal mature BDNF in astrocytes 6
3.2 Optimal QD-mBDNF treatment condition for observing endosomal BDNF 6
3.3 Endosomal pathways processing QD-mBDNF in astrocytes 7
3.4 Transport of endocytosed QD-mBDNF is affected by increase in intracellular Ca2+ 8
IV. Discussion 9
V. Figure legends 11
VI. Figures 14
References 26
Abstract in Korea 28
- URI
-
http://dgist.dcollection.net/common/orgView/200000171531
http://hdl.handle.net/20.500.11750/10700
- Degree
- MASTER
- Department
- Brain and Cognitive Sciences
- Publisher
- DGIST
- Related Researcher
-
-
Choe, Han Kyoung
- Research Interests Modulation of neural circuit; Circadian regulation of behavior and perception; Neurotechnology
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- Files in This Item:
-
There are no files associated with this item.
- Appears in Collections:
- Department of Brain Sciences Theses Master
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