Presenilin-2 (PS2) mediates corticosterone-induced autophagic cell death in adult hippocampal neural stem cells
- Title
- Presenilin-2 (PS2) mediates corticosterone-induced autophagic cell death in adult hippocampal neural stem cells
- Author(s)
- Jihyun Hong
- DGIST Authors
- Yu, Seong-Woon ; Hong, Jihyun ; Jang, Jae Eun
- Advisor
- 유성운
- Co-Advisor(s)
- Jae-Eun Jang
- Issued Date
- 2019
- Awarded Date
- 2019-02
- Type
- Thesis
- Table Of Contents
-
Ⅰ. Introduction 1
Ⅱ. Material and Methods 7
2.1 Reagents and antibodies 7
2.2 Cell culture 8
2.3 Construction of CRISPR/Cas9-mediated KO cells 8
2.4 Transfection, Expression vectors 9
2.5 Cell death assay 9
2.6 Western blotting 10
2.7 Quantitative real-time polymerase chain reaction (qRT-PCR) 11
2.8 Statistical analysis 12
Ⅲ. Results 13
3.1 CORT increases ACD, not apoptosis in HCN stem cells 13
3.2 CORT increases PS2 expression in HCN stem cells 15
3.3 Knockout of PS2 prevents CORT-induced ACD 17
3.4 The rescue of PS2 increases CORT-induced ACD 19
3.5 Autophagy initiation step is intact in PS2 KO 20
3.6 LC3 modification is impaired by PS2 KO 23
Ⅳ. Discussion 26
Ⅴ. Reference 30
Abstract in Korean 36
- URI
-
http://dgist.dcollection.net/common/orgView/200000171535
http://hdl.handle.net/20.500.11750/10703
- Degree
- MASTER
- Department
- Brain and Cognitive Sciences
- Publisher
- DGIST
- Related Researcher
-
-
Yu, Seong-Woon
- Research Interests Molecular mechanisms of neuronal cell death and neurodegeneration
-
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- Appears in Collections:
- Department of Brain Sciences Theses Master
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