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Flavonoids have modulatory effects on neuroinflammation in microglia

Title
Flavonoids have modulatory effects on neuroinflammation in microglia
Translated Title
미세아교세포에서 flavonoid 의 신경염증 억제 효과
Authors
Cho, Hyo Jin
DGIST Authors
Cho, Hyo Jin; Yu, Seong Woon; Choi, Hong Soo
Advisor(s)
Yu, Seong Woon
Co-Advisor(s)
Choi, Hong Soo
Issue Date
2015
Available Date
2015-01-12
Degree Date
2015. 2
Type
Thesis
Keywords
flavonoidneuroinflammationmicrogliaNF-κB신경염증미세아교세포
Abstract
Microglia are the resident macrophages in the central nervous system. Under normal conditions, microglia cells contribute to brain development and maintenance of tissue homeostasis. However, chronic microglial activation can cause tissue damage and eventually lead to neurodegenerative diseases and neuronal death. In terms of phenotype,activated microglia can be divided into two different types: M1 state and M2 state. The first is classical activation, M1 state, which is typified by the production of inflammatory cytokines and reactive oxygen species (ROS). In contrast with pro-inflammatory M1 cells, alternative activation, M2 state has an anti-inflammatory phenotype promoting wound repair and debris clearance.Recently, the effects of flavonoids on a variety of inflammatory processes and immune functions have been reported. Flavonoids, which are plant pigments, are anti-oxidants that possess anti-inflammatory properties and may modulate signaling pathways. This study aims to investigate the anti-inflammatory effect of flavonoids and their role on the NF-kB pathway in BV2 microglial cells. Flavonoids treatment suppress production of pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α), nitric oxide (NO) and interleukin-6 (IL-6) in lipopolysaccharide (LPS)-stimulated BV2 cells. Flavonoids treatment also attenuated activation of nuclear factor-kappaB (NF-κB) transcriptional activity. Furthermore,flavonoids suppress mRNA expressions related with pro-inflammatory M1 state. These observations suggest that flavonoids may contribute as an anti-inflammatory agent and provide evidence about its role in the prevention of neurodegenerative diseases associated with inflammation. ⓒ 2015 DGIST
Table Of Contents
I. INTRODUCTION 1-- 1. 1. Introduction 1-- II. Methods and Materials 3-- 2. 1. Chemicals and reagents 3-- 2. 2. Cell culture 3-- 2. 3. Cell viability 3-- 2. 4.Nitrite assay 4-- 2. 5.ELISA 4-- 2. 6. NF-kB luciferase reporter assay 4-- 2. 7. RT-PCR 5-- 2. 8. Statistical analyses 5-- III. Results 7-- 3. 1. Flavonoids reduce NO production in LPS-stimulated BV2 microglia 7-- 3. 2. Flavonoids number 1, 53, 61 do not affect cell viability in LPS-stimulated BV2 microglia 7-- 3. 3. Flavonoids suppress production of inflammatory cytokines in LPS-stimulated BV2 microglia 13-- 3. 4. Flavonoids blocks NF- ĸB activity in LPS-stimulated BV2 microglia 15-- 3. 5. Flavonoids affect production of mRNA levels in M1, M2 state 17-- IV. Discussion 22-- V. References 24
URI
http://dgist.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000001923092
http://hdl.handle.net/20.500.11750/1399
DOI
10.22677/thesis.1923092
Degree
Master
Department
Brain Science
University
DGIST
Files:
Collection:
Brain and Cognitive SciencesThesesMaster


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