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Characterization of Interaction Between Pathogenic PolyQ Proteins and Q-rich Target Proteins

Title
Characterization of Interaction Between Pathogenic PolyQ Proteins and Q-rich Target Proteins
Translated Title
폴리글루타민 단백질과 글루타민을 많이 포함하는 표적 단백질 간의 상호작용의 특성 분석
Authors
Kim, Gyu Ree
DGIST Authors
Kim, Gyu Ree; Lee, Sung Bae; Kim, Kee Tae
Advisor(s)
Lee, Sung Bae
Co-Advisor(s)
Kim, Kee Tae
Issue Date
2016
Available Date
2016-02-12
Degree Date
2016. 2
Type
Thesis
Keywords
Polyglutamine diseaseQ-rich proteinstarget proteinsinteractionglue theory폴리글루타민 질병글루타민을 많이 갖는 단백질표적 단백질상호작용접착제 작용 이론
Abstract
To understand the pathogenesis of many diseases attributed to protein toxicity such as polyglutamine (polyQ) diseases, it is very crucial to determine how these toxic disease proteins interact and trap their numerous targets. However, in polyQ diseases, details of required features for their interaction with targets remain largely unknown. Here, we identified what features are necessity for interaction between polyQ and targets, and how target proteins have an effect on polyQ aggregate formation. We visualized the interaction between pathogenic polyQ proteins and Q-rich possible target proteins in neurons, which is predicted on the Q-Q based protein interaction occurring in pathogenic polyQ-containing proteins for their self-oligomerization/aggregation. Furthermore, we checked whether the interaction of pathogenic polyQ proteins with targets can be modulated through designed strategies such as using a structural inhibitor molecule. Through this study, we established the model system to study the modes of interaction between pathogenic polyQ and Q-rich target proteins and aim to prove the possibility of target proteins accelerating aggregate formation by acting as glue. ⓒ 2016 DGIST
Table Of Contents
Ⅰ. INTRODUCTION 1 -- Ⅱ. MATERIALS AND METHOD 3 -- 2.1 Fly stocks 3 -- 2.2 Identification of Q-rich proteins 3 -- 2.3 Immunostaining 4 -- 2.4 Statistical analysis for significant test 5 -- 2.5 Microscope Imaging 5 -- Ⅲ. RESULT 6 -- 3.1 Pathogenic polyQ proteins interact with Q-rich polyQ proteins 6 -- 3.2 Possible target proteins in the cell and Co-lacaliztion between possible Q-rich target proteins and pathogenic polyQ proteins 8 -- 3.3 Overexpressed target proteins with polyQ proteins increased aggregation form 10 -- 3.4 QBP1 prevent interaction between polyQ proteins and target proteins 11 -- 3.5 RNA interference of target proteins decreased polyQ protein (Htt) aggregate numbers 13 -- IV. DISCUSSION 14 -- V. Figures and tables 15 -- VI. References 39 -- VII. Summary in Korean 43
URI
http://dgist.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000002230561
http://hdl.handle.net/20.500.11750/1428
DOI
10.22677/thesis.2230561
Degree
Master
Department
Brain and Cognitive Sciences
University
DGIST
Files:
Collection:
Brain and Cognitive SciencesThesesMaster


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