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ASIC2a-dependent increase of ASIC3 surface expression enhances the sustained component of the currents

Title
ASIC2a-dependent increase of ASIC3 surface expression enhances the sustained component of the currents
Authors
Kweon, HJ[Kweon, Hae-Jin]Cho, JH[Cho, Jin-Hwa]Jang, IS[Jang, Il-Sung]Suh, BC[Suh, Byung-Chang]
DGIST Authors
Kweon, HJ[Kweon, Hae-Jin]; Suh, BC[Suh, Byung-Chang]
Issue Date
2016-10-31
Citation
BMB Reports, 49(10), 542-547
Type
Article
Article Type
Article
Keywords
Acid-Sensing Ion ChannelEndoplasmic ReticulumHeteromeric AssemblyMembrane ProteinSurface Trafficking
ISSN
1976-6696
Abstract
Acid-sensing ion channels (ASICs) are proton-gated cation channels widely expressed in the nervous system. Proton sensing by ASICs has been known to mediate pain, mechanosensation, taste transduction, learning and memory, and fear. In this study, we investigated the differential subcellular localization of ASIC2a and ASIC3 in heterologous expression systems. While ASIC2a targeted the cell surface itself, ASIC3 was mostly accumulated in the ER with partial expression in the plasma membrane. However, when ASIC3 was co-expressed with ASIC2a, its surface expression was markedly increased. By using bimolecular fluorescence complementation (BiFC) assay, we confirmed the heteromeric association between ASIC2a and ASIC3 subunits. In addition, we observed that the ASIC2a-dependent surface trafficking of ASIC3 remarkably enhanced the sustained component of the currents. Our study demonstrates that ASIC2a can increase the membrane conductance sensitivity to protons by facilitating the surface expression of ASIC3 through herteromeric assembly. © 2016 by the The Korean Society for Biochemistry and Molecular Biology.
URI
http://hdl.handle.net/20.500.11750/1539
DOI
10.5483/BMBRep.2016.49.10.057
Publisher
Korean Society for Molecular and Cellular Biology
Related Researcher
Files:
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Collection:
Brain and Cognitive SciencesETC1. Journal Articles


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