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Acceleration of reaction in charged microdroplets

Title
Acceleration of reaction in charged microdroplets
Authors
Lee, JK[Lee, Jae Kyoo]Banerjee, S[Banerjee, Shibdas]Nam, HG[Nam, Hong Gil]Zare, RN[Zare, Richard N.]
DGIST Authors
Nam, HG[Nam, Hong Gil]
Issue Date
2015-11
Citation
Quarterly Reviews of Biophysics, 48(4), 437-444
Type
Article
Article Type
Review
Keywords
2,6-Dichloroindophenol2,6 DichlorophenolindophenolAccelerationAerosolAerosolsAnimalAnimalsAscorbic AcidBiophysical PhenomenaBiophysicsCardiac MuscleChemistryCytochrome CCytochromes CDroplet FusionElectospray IonizationElectrospray Mass SpectrometryHorseHorsesIsoquinolineKineticsMacromolecular SubstancesMacromoleculeMaltoseMass SpectrometryMetabolismMyocardiumProceduresSolution and SolubilitySolutionsSolventSolventsSpectrometry, Mass, Electrospray IonizationWater
ISSN
0033-5835
Abstract
Using high-resolution mass spectrometry, we have studied the synthesis of isoquinoline in a charged electrospray droplet and the complexation between cytochrome c and maltose in a fused droplet to investigate the feasibility of droplets to drive reactions (both covalent and noncovalent interactions) at a faster rate than that observed in conventional bulk solution. In both the cases we found marked acceleration of reaction, by a factor of a million or more in the former and a factor of a thousand or more in the latter. We believe that carrying out reactions in microdroplets (about 1-15 μm in diameter corresponding to 0·5 pl-2 nl) is a general method for increasing reaction rates. The mechanism is not presently established but droplet evaporation and droplet confinement of reagents appear to be two important factors among others. In the case of fused water droplets, evaporation has been shown to be almost negligible during the flight time from where droplet fusion occurs and the droplets enter the heated capillary inlet of the mass spectrometer. This suggests that (1) evaporation is not responsible for the acceleration process in aqueous droplet fusion and (2) the droplet-air interface may play a significant role in accelerating the reaction. We argue that this 'microdroplet chemistry' could be a remarkable alternative to accelerate slow and difficult reactions, and in conjunction with mass spectrometry, it may provide a new arena to study chemical and biochemical reactions in a confined environment. © 2015 Cambridge University Press.
URI
http://hdl.handle.net/20.500.11750/1563
DOI
10.1017/S0033583515000086
Publisher
CAMBRIDGE UNIV PRESS
Related Researcher
Files:
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Collection:
New BiologyETC1. Journal Articles


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