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Genetic Identification of ACC-RESISTANT2 Reveals Involvement of LYSINE HISTIDINE TRANSPORTER1 in the Uptake of 1-Aminocyclopropane-1-Carboxylic Acid in Arabidopsis thaliana

Title
Genetic Identification of ACC-RESISTANT2 Reveals Involvement of LYSINE HISTIDINE TRANSPORTER1 in the Uptake of 1-Aminocyclopropane-1-Carboxylic Acid in Arabidopsis thaliana
Authors
Shin, K[Shin, Kihye]Lee, S[Lee, Sumin]Song, WY[Song, Won-Yong]Lee, RA[Lee, Rin-A]Lee, I[Lee, Inhye]Ha, K[Ha, Kyungsun]Koo, JC[Koo, Ja-Choon]Park, SK[Park, Soon-Ki]Nam, HG[Nam, Hong-Gil]Lee, Y[Lee, Youngsook]Soh, MS[Soh, Moon-Soo]
DGIST Authors
Nam, HG[Nam, Hong-Gil]
Issue Date
2015-03
Citation
Plant and Cell Physiology, 56(3), 572-582
Type
Article
Article Type
Article
ISSN
0032-0781
Abstract
1-Aminocyclopropane-1-carboxylic acid (ACC) is a biosynthetic precursor of ethylene, a gaseous plant hormone which controls a myriad of aspects of development and stress adaptation in higher plants. Here, we identified a mutant in Arabidopsis thaliana, designated as ACC-resistant2 (are2), displaying a dose-dependent resistance to exogenously applied ACC. Physiological analyses revealed that mutation of are2 impaired various aspects of exogenous ACC-induced ethylene responses, while not affecting sensitivity to other plant hormones during seedling development. Interestingly, the are2 mutant was normally sensitive to gaseous ethylene, compared with the wild type. Double mutant analysis showed that the ethylene-overproducing mutations, eto1 or eto3, and the constitutive ethylene signaling mutation, ctr1 were epistatic to the are2 mutation. These results suggest that the are2 mutant is not defective in ethylene biosynthesis or ethylene signaling per se. Map-based cloning of ARE2 demonstrated that LYSINE HISTIDINE TRANSPORTER1 (LHT1), encoding an amino acid transporter, is the gene responsible. An uptake experiment with radiolabeled ACC indicated that mutations of LHT1 reduced, albeit not completely, uptake of ACC. Further, we performed an amino acid competition assay and found that two amino acids, alanine and glycine, known as substrates of LHT1, could suppress the ACC-induced triple response in a LHT1-dependent way. Taken together, these results provide the first molecular genetic evidence supporting that a class of amino acid transporters including LHT1 takes part in transport of ACC, thereby influencing exogenous ACC-induced ethylene responses in A. thaliana.
URI
http://hdl.handle.net/20.500.11750/1576
DOI
10.1093/pcp/pcu201
Publisher
Oxford University Press
Related Researcher
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Collection:
New BiologyETC1. Journal Articles


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