Identification and characterization of a MYB transcription factor, AT3G as a positive regulator of leaf senescence in Arabidopsis
- Title
- Identification and characterization of a MYB transcription factor, AT3G as a positive regulator of leaf senescence in Arabidopsis
- Author(s)
- Daesang You
- DGIST Authors
- Daesang You ; Hye Ryun Woo ; Chang-Hun Lee
- Advisor
- 우혜련
- Co-Advisor(s)
- Chang-Hun Lee
- Issued Date
- 2022
- Awarded Date
- 2022/02
- Type
- Thesis
- Description
- MYB transcription factor, Leaf senescence, Anthocyanin, Arabidopsis
- Table Of Contents
-
Ⅰ. Introduction 1
1.1 Leaf senescence 1
1.2 Anthocyanin accumulation 1
1.3 Relationship between anthocyanin accumulation and leaf senescence 2
Ⅱ. Methods 4
2.1 Plant materials and growth conditions 4
2.2 Leaf senescence assays 4
2.3 Measurement of chlorophyll and anthocyanin content 4
2.4 RNA extraction 4
2.5 Gene expression analysis 4
2.6 Split luciferase assay 5
Ⅲ. Results 6
3.1 Identification of a candidate transcription factor, AT3G 6
3.2 Dark-induced leaf senescence phenotypes of AT3G-KO and AT3G-OX leaves 8
3.3 Phenotypes of AT3G-KO and AT3G-OX under HL stress condition 9
3.4 Protein-protein interaction between AT3G and PAP1 12
Ⅳ. Discussion 13
- URI
-
http://dgist.dcollection.net/common/orgView/200000597133
http://hdl.handle.net/20.500.11750/16288
- Degree
- Master
- Department
- New Biology
- Publisher
- DGIST
- Related Researcher
-
-
Woo, Hye Ryun
- Research Interests Genome biology; Epigenomics; Plant senescence
-
- Files in This Item:
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- Appears in Collections:
- Department of New Biology Theses Master
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