Cited 4 time in webofscience Cited 5 time in scopus

Spatial and temporal coordination of insulin granule exocytosis in intact human pancreatic islets

Title
Spatial and temporal coordination of insulin granule exocytosis in intact human pancreatic islets
Authors
Almaca, J[Almaca, Joana]Liang, T[Liang, Tao]Gaisano, HY[Gaisano, Herbert Y.]Nam, HG[Nam, Hong Gil]Berggren, PO[Berggren, Per-Olof]Caicedo, A[Caicedo, Alejandro]
DGIST Authors
Almaca, J[Almaca, Joana]; Nam, HG[Nam, Hong Gil]
Issue Date
2015-12
Citation
Diabetologia, 58(12), 2810-2818
Type
Article
Article Type
Article
Keywords
AdultBody MassCell IsolationCell PopulationConcentration ResponseConfocal MicroscopyCoordinationCytoplasmExocytosisFemaleFluorescent DyeGlucoseGlucose HomeostasisGreen Fluorescent ProteinHumanHuman CellHuman ExperimentHuman IsletsIn Vivo StudyInsulinInsulin GranuleInsulin ReleaseLong Term ExposureMaleNeuropeptide YPancreas IsletPriority JournalProtein SecretionPulsatile SecretionSecretory GranuleSomatostatinSpatial and Temporal CoordinationSpatiotemporal Analysis
ISSN
0012-186X
Abstract
Aims/hypothesis: Insulin secretion is widely studied because it plays a central role in glucose homeostasis and diabetes. Processes from insulin granule fusion in beta cells to in vivo insulin secretion have been elucidated, but data at the cellular level do not fully account for several aspects of the macroscopic secretory pattern. Here we investigated how individual secretory events are coordinated spatially and temporally within intact human islets. Methods: We used the fluorescent probe neuropeptide Y (NPY)–pHluorin to visualise insulin granule secretion in isolated intact human islets. Results: We found that individual beta cells respond to increases in glucose concentration by releasing insulin granules in very discrete bursts with periods consistent with in vivo pulsatile insulin secretion. In successive secretory bursts during prolonged exposure to high glucose levels, secretory events progressively localised to preferential release sites, coinciding with the transition to second phase insulin secretion. Granule secretion was very synchronised in neighbouring beta cells, forming discrete regional clusters of activity. Conclusions/interpretation: These results reveal how individual secretory events are coordinated to produce pulsatile insulin secretion from human islets. © 2015, Springer-Verlag Berlin Heidelberg.
URI
http://hdl.handle.net/20.500.11750/1654
DOI
10.1007/s00125-015-3747-9
Publisher
Springer
Related Researcher
Files:
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Collection:
New BiologyCBRG(Complex Biology Research Group)1. Journal Articles


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