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SU-8-based nanoporous substrate for migration of neuronal cells

Title
SU-8-based nanoporous substrate for migration of neuronal cells
Authors
Kim, E[Kim, Eunhee]Yoo, SJ[Yoo, Seung-Jun]Moon, C[Moon, Cheil]Nelson, BJ[Nelson, Bradley J.]Choi, H[Choi, Hongsoo]
DGIST Authors
Kim, E[Kim, Eunhee]; Yoo, SJ[Yoo, Seung-Jun]; Moon, C[Moon, Cheil]; Nelson, BJ[Nelson, Bradley J.]; Choi, H[Choi, Hongsoo]
Issue Date
2015-06-15
Citation
Microelectronic Engineering, 141, 173-177
Type
Article
Article Type
Article; Proceedings Paper
Keywords
Amino AcidsCell CultureCell MobilityCell ViabilityCellsCytologyFunctional PolymersMobile SecurityNano-Porous SurfaceNano-Porous SurfacesNanoporesNanosphere Lithography (NSl)NeuronsPhotoresistsPorous MaterialsSU-8Substrates
ISSN
0167-9317
Abstract
Most polymer-based biomedical implantable microscale devices have a smooth surface, so that cell seeding is suppressed in the absence of an adhesive material coating on the surface. SU-8 is a negative photoresist, and is widely used for the fabrication of micro-/nanoscale biomedical devices. A physical surface modification technique was introduced in this study to enhance cell viability and mobility on a SU-8 substrate. To characterize cell viability and mobility, four types of SU-8 substrate were prepared: flat bare substrate, poly-l-lysine (PLL)-coated flat bare substrate, nanoporous substrate, and PLL-coated nanoporous substrate. Rat pheochromocytoma (PC12) cells were cultured on these substrates, and nerve growth factor (NGF) was added to induce differentiation of the PC12 cells. On the seventh day of cell culture, PC12 cells on the nanoporous SU-8 substrate showed 24.3% cell differentiation (neurite outgrowth) versus 1.1% cell differentiation on the flat bare substrate. It was also found that cells had a tendency to move from a flat surface to a nanoporous region. These cellular activities on the nanoporous SU-8 substrate suggest that nanopores can be used to regulate cellular activities and can be applied to SU-8-based microscale biomedical devices. © 2015 Elsevier B.V. All rights reserved.
URI
http://hdl.handle.net/20.500.11750/1660
DOI
10.1016/j.mee.2015.03.016
Publisher
Elsevier B.V.
Related Researcher
Files:
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Collection:
Brain and Cognitive SciencesMoon Lab1. Journal Articles
Robotics EngineeringETC1. Journal Articles
Robotics EngineeringETC1. Journal Articles


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