Development of miniaturized Microfluidic Electrochemical Nano-Biosensor platforms for the detection of various Biomarkers.
- Title
- Development of miniaturized Microfluidic Electrochemical Nano-Biosensor platforms for the detection of various Biomarkers.
- Alternative Title
- 다양한 바이오 마커 검출을 위한 미세 유체 전기 화학 나노 바이오 센서 플랫폼 개발
- Author(s)
- KRISHNA CHAITANYA SATISH BABU KASTURI
- DGIST Authors
- KRISHNA CHAITANYA SATISH BABU KASTURI ; CheolGi Kim ; Jong-Sung Yu
- Advisor
- 김철기
- Co-Advisor(s)
- Jong-Sung Yu
- Issued Date
- 2021
- Awarded Date
- 2021/02
- Type
- Thesis
- Table Of Contents
-
1. Introduction 1
1.1. Microfluidics 2
1.2. Electrochemistry 3
1.3. Biosensors 8
1.4. Microfluidic electrochemical biosensing 14
2. Electrochemical biosensor based on naturally reduced rGO/Au nanocomposite for the sensitive detection of the miRNA-122 biomarker 18
2.1. Introduction 18
2.2. Experimental details of the biosensor 20
2.2.1. Materials and chemicals 20
2.2.2. Instruments required for biosensing of miRNA-122 20
2.2.3. Synthesis of rGO/Au nanocomposite 21
2.2.4. Fabrication of rGO/Au modified electrode 22
2.2.5. Probe-DNA immobilization and target miRNA hybridization 22
2.2.6. Electrochemical measurement 23
2.3. Results and discussion 24
2.3.1. Characterization of rGO/Au nanocomposites 24
2.3.2. Time optimization for immobilization and hybridization 28
2.3.3. Immobilization and hybridization studies 29
2.3.4. DPV detection of miRNA-122 biomarker 31
2.3.5. Biomarker selectivity test 32
2.4. Stability and reproducibility of the biosensor 33
2.5. Conclusions 35
3. Experimental techniques and fabrication of microfluidic electrochemical biosensors 37
3.1. Materials required for the fabrication of microfluidic electrochemical biosensors 37
3.1.1. Lithography of Microfluidic compartments 37
3.1.2. Pneumatic microvalve fabrication 38
3.1.3. Microfluidic channel fabrication 38
3.1.4. Electrodes Fabrication 38
3.2. Characterization techniques 40
3.2.1. Scanning Electron Microscope (SEM) 41
3.2.2. Atomic Force microscope (AFM) 42
3.2.3. Autolab 42
3.3. Experimental setup 42
3.4. Lab-on-a-chip-based electrochemical device for the detection beta-amyloid detection 44
4. Lab-on-a-chip based electrochemical device for simultaneous detection of multiple biomarkers 58
4.1. Construction of the miniaturized electrochemical device 59
4.2. Lab-on-a-chip based twelve electrode multiplex analysis for the detection of multiple biomarkers 63
4.3. Surface-enhanced electrode biosensors 66
Conclusion 68
References 70
List of Publications 86
Korean abstract(요약문) 87
- URI
-
http://dgist.dcollection.net/common/orgView/200000364302
http://hdl.handle.net/20.500.11750/16690
- Degree
- Doctor
- Department
- Emerging Materials Science
- Publisher
- DGIST
- Related Researcher
-
-
Kim, CheolGi
- Research Interests Magnetic Materials and Spintronics; Converging Technology of Nanomaterials and Biomaterials; Bio-NEMS;MEMS
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- Appears in Collections:
- Department of Physics and Chemistry Theses Ph.D.
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