Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Kwon, J. | - |
dc.contributor.author | Ko, E. | - |
dc.contributor.author | Cho, S.-Y. | - |
dc.contributor.author | Lee, Y.-H. | - |
dc.contributor.author | Jun, S. | - |
dc.contributor.author | Lee, K. | - |
dc.contributor.author | Hwang, E. | - |
dc.contributor.author | Vaidya, B. | - |
dc.contributor.author | Hwang, J.-H. | - |
dc.contributor.author | Kim, N. | - |
dc.contributor.author | Song, M.-K. | - |
dc.contributor.author | Kim, H.-Y. | - |
dc.contributor.author | Ito, Dai | - |
dc.contributor.author | Lin, Y. | - |
dc.contributor.author | Jo, E. | - |
dc.contributor.author | Yang, K.E. | - |
dc.contributor.author | Chung, H.-C. | - |
dc.contributor.author | Cha, S. | - |
dc.contributor.author | Kim, D.I. | - |
dc.contributor.author | Yi, Y.-S. | - |
dc.contributor.author | Yun, S.-H. | - |
dc.contributor.author | Park, S.C. | - |
dc.contributor.author | Lee, S. | - |
dc.contributor.author | Choi, J.-S. | - |
dc.contributor.author | Kim, D.S. | - |
dc.contributor.author | Kimc, D. | - |
dc.date.accessioned | 2022-11-15T11:40:10Z | - |
dc.date.available | 2022-11-15T11:40:10Z | - |
dc.date.created | 2022-03-14 | - |
dc.date.issued | 2022-02 | - |
dc.identifier.issn | 2165-0497 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/17125 | - |
dc.description.abstract | The antigen-based rapid diagnostic test (Ag-RDT) using saliva specimens is fast, noninvasive, and suitable for SARS-CoV-2 self-testing, unlike nasopharyngeal swab (NPS) testing. We evaluated a novel Beanguard gargle (BG)-based virus collection method that can be applied to Ag-RDT as an alternative to the current RT-PCR with an NPS for early diagnosis of COVID-19. This clinical trial comprised 102 COVID-19-positive patients hospitalized after a governmental screening process and 100 healthy individuals. Paired NPS and BG-based saliva specimens from COVID-19 patients and healthy individuals were analyzed using NPS-RT-PCR, BG-RT-PCR, and BG-Ag-RDTs, whose diagnostic performance for detecting SARS-CoV-2 was compared. BG-Ag-RDTs showed high sensitivity (97.8%) and specificity (100%) in 45 patients within 6 days of illness and detected all cases of SARS-CoV-2 Alpha and Delta variants. In 11 asymptomatic active COVID-19 cases, both BG-Ag-RDTs and BG-RT-PCR showed sensitivities and specificities of 100%. Sensitivities of BG-Ag-RDT and BG-RT-PCR toward salivary viral detection were highly concordant, with no discrimination between symptomatic (97.0%), asymptomatic (100%), or SARS-CoV-2 variant (100%) cases. The intermolecular interactions between SARS-CoV-2 spike proteins and truncated canavalin, an active ingredient from the bean extract (BE), were observed in terms of physicochemical properties. The detachment of the SARS-CoV-2 receptor-binding domain from hACE2 increased as the BE concentration increased, allowing the release of the virus from hACE2 for early diagnosis. Using BG-based saliva specimens remarkably enhances the Ag-RDT diagnostic performance as an alternative to NPS and enables noninvasive, rapid, and accurate COVID-19 self-testing and mass screening, supporting efficient COVID-19 management. © 2022 American Society for Microbiology. All rights reserved. | - |
dc.language | English | - |
dc.publisher | American Society for Microbiology | - |
dc.title | Bean Extract-Based Gargle for Efficient Diagnosis of Active COVID-19 Infection Using Rapid Antigen Tests | - |
dc.type | Article | - |
dc.identifier.doi | 10.1128/spectrum.01614-21 | - |
dc.identifier.scopusid | 2-s2.0-85125212602 | - |
dc.identifier.bibliographicCitation | Microbiology Spectrum, v.10, no.1 | - |
dc.description.isOpenAccess | TRUE | - |
dc.subject.keywordAuthor | COVID-19 | - |
dc.subject.keywordAuthor | Oral virus | - |
dc.subject.keywordAuthor | Rapid diagnostic test | - |
dc.subject.keywordAuthor | SARS-CoV-2 | - |
dc.subject.keywordAuthor | Sensitivity | - |
dc.subject.keywordAuthor | Specificity | - |
dc.citation.number | 1 | - |
dc.citation.title | Microbiology Spectrum | - |
dc.citation.volume | 10 | - |
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