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Real-time detection of neurite outgrowth using microfluidic device

Title
Real-time detection of neurite outgrowth using microfluidic device
Authors
Kim, Sam HwanJang, Jong MoonChoi, Hong SooMoon, Che Il
DGIST Authors
Kim, Sam Hwan; Jang, Jong Moon; Choi, Hong SooMoon, Che Il
Issue Date
2013
Citation
SPIE Nano-Bio Sensing, Imaging, and Spectroscopy, 8879
Type
Conference
Article Type
Conference Paper
ISBN
9780000000000
ISSN
0277-786X
Abstract
We developed a simple method for real-time detection of the neurite outgrowth using microfluidic device. Our microfluidic device contains three compartmentalized channels which are for cell seeding, hydrogel and growth factors. Collagen gel is filled in the middle channel and pheochromocytoma (PC12) cells are seeded in the left channel. To induce differentiation of PC12 cells, 50 ng/ml to1000 ng/ml of nerve growth factor (NGF) is introduced into the right channel. After three days of NGF treatment, PC12 cells begin to extend neurites and formed neurite network from sixth day. Quantification of neurite outgrowth is analyzed by measuring the total area of neurites. On sixth day, the area is doubled compared to the area on third day and increases by 20 times on ninth day. © 2013 SPIE.
URI
http://hdl.handle.net/20.500.11750/1780
DOI
10.1117/12.2018667
Publisher
SPIE
Related Researcher
Files:
There are no files associated with this item.
Collection:
Robotics EngineeringBio-Micro Robotics Lab2. Conference Papers
Robotics EngineeringETC2. Conference Papers


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