Cited 3 time in webofscience Cited 3 time in scopus

The Evolutionarily Conserved LIM Homeodomain Protein LIM-4/LHX6 Specifies the Terminal Identity of a Cholinergic and Peptidergic C. elegans Sensory/Inter/Motor Neuron-Type

Title
The Evolutionarily Conserved LIM Homeodomain Protein LIM-4/LHX6 Specifies the Terminal Identity of a Cholinergic and Peptidergic C. elegans Sensory/Inter/Motor Neuron-Type
Authors
Kim, J[Kim, Jinmahn]Yeon, J[Yeon, Jihye]Choi, SK[Choi, Seong-Kyoon]Huh, YH[Huh, Yang Hoon]Fang, Z[Fang, Zi]Park, SJ[Park, Seo Jin]Kim, MO[Kim, Myoung Ok]Ryoo, ZY[Ryoo, Zae Young]Kang, K[Kang, Kyeongjin]Kweon, HS[Kweon, Hee-Seok]Jeon, WB[Jeon, Won Bae]Li, C[Li, Chris]Kim, K[Kim, Kyuhyung]
DGIST Authors
Kim, J[Kim, Jinmahn]; Yeon, J[Yeon, Jihye]; Choi, SK[Choi, Seong-Kyoon]; Fang, Z[Fang, Zi]; Jeon, WB[Jeon, Won Bae]Kim, K[Kim, Kyuhyung]
Issue Date
2015-08
Citation
PLoS Genetics, 11(8)
Type
Article
Article Type
Article
Keywords
Caenorhabditis ElegansCell FateControlled StudyDNA SequenceFlp 12 Neuropeptide GeneGeneGene Expression RegulationGenetic VariabilityHumanHuman CellInterneuronLHX6 ProteinLHX8 ProteinLIM 4 ProteinLIM ProteinMarker GeneMolecular EvolutionMotoneuronNeuroblastoma CellNon-HumanProtein DomainProtein FunctionProtein MotifProtein TargetingSensory Nerve CellTranscription FactorUnclassified Drug
ISSN
1553-7390
Abstract
The expression of specific transcription factors determines the differentiated features of postmitotic neurons. However, the mechanism by which specific molecules determine neuronal cell fate and the extent to which the functions of transcription factors are conserved in evolution are not fully understood. In C. elegans, the cholinergic and peptidergic SMB sensory/inter/motor neurons innervate muscle quadrants in the head and control the amplitude of sinusoidal movement. Here we show that the LIM homeobox protein LIM-4 determines neuronal characteristics of the SMB neurons. In lim-4 mutant animals, expression of terminal differentiation genes, such as the cholinergic gene battery and the flp-12 neuropeptide gene, is completely abolished and thus the function of the SMB neurons is compromised. LIM-4 activity promotes SMB identity by directly regulating the expression of the SMB marker genes via a distinct cis-regulatory motif. Two human LIM-4 orthologs, LHX6 and LHX8, functionally substitute for LIM-4 in C. elegans. Furthermore, C. elegans LIM-4 or human LHX6 can induce cholinergic and peptidergic characteristics in the human neuronal cell lines. Our results indicate that the evolutionarily conserved LIM-4/LHX6 homeodomain proteins function in generation of precise neuronal subtypes. © 2015 Kim et al.
URI
http://hdl.handle.net/20.500.11750/2337
DOI
10.1371/journal.pgen.1005480
Publisher
Public Library of Science
Related Researcher
Files:
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Collection:
Division of Nano∙Energy Convergence Research1. Journal Articles
Brain and Cognitive SciencesETC1. Journal Articles


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