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TLR2-induced astrocyte MMP9 activation compromises the blood brain barrier and exacerbates intracerebral hemorrhage in animal models

Title
TLR2-induced astrocyte MMP9 activation compromises the blood brain barrier and exacerbates intracerebral hemorrhage in animal models
Authors
Min, H[Min, Hyunjung]Hong, J[Hong, Jinpyo]Cho, IH[Cho, Ik-Hyun]Jang, YH[Jang, Yong Ho]Lee, H[Lee, Hyunkyoung]Kim, D[Kim, Dongwoon]Yu, SW[Yu, Seong-Woon]Lee, S[Lee, Soojin]Lee, SJ[Lee, Sung Joong]
DGIST Authors
Yu, SW[Yu, Seong-Woon]
Issue Date
2015-04-10
Citation
Molecular Brain, 8
Type
Article
Article Type
Article
Keywords
AnimalAnimal ExperimentAnimal ModelAnimal TissueAnimaliaAnimalsAstrocyteAstrocytesBlood-Brain BarrierBlood Brain BarrierBrain HemorrhageBrain TissueC57Bl MouseCerebral HemorrhageChemokineChemokinesCollagenaseCollagenasesControlled StudyDeficiencyDisease CourseDisease ExacerbationDisease ModelDisease Models, AnimalDisease ProgressionEnzyme ActivationEnzyme RegulationEnzymologyGelatinase BIntercellular Adhesion Molecule-1Knockout MouseMaleMatrix Metalloproteinase-9MetabolismMice, Inbred C57BLMice, KnockoutMouseMusNeuroinflammationNeutrophilNeutrophil ChemotaxisNeutrophil InfiltrationNon-HumanPathologyPriority JournalProtein ExpressionSignal TransductionStrokeToll-Like ReceptorToll-Like Receptor2
ISSN
1756-6606
Abstract
Background: The innate immune response plays an important role in the pathogenesis of intracerebral hemorrhage (ICH). Recent studies have shown that Toll-like receptor 2 (TLR2) is involved in the innate immune response in various neurological diseases, yet neither its role in ICH nor the mechanisms by which it functions have yet been elucidated. We examined these in this study using a collagenase-induced mouse ICH model with TLR2 knock-out (KO) mice. Results: TLR2 expression was upregulated in the ipsilateral hemorrhagic tissues of the collagenase-injected mice. Brain injury volume and neurological deficits following ICH were reduced in TLR2 KO mice compared to wild-type (WT) control mice. Heterologous blood-transfer experiments show that TLR2 signaling in brain-resident cells, but not leukocytes, contributes to the injury. In our study to elucidate underlying mechanisms, we found that damage to blood-brain barrier (BBB) integrity following ICH was attenuated in TLR2 KO mice compared to WT mice, which may be due to reduced matrix metalloproteinase-9 (MMP9) activation in astrocytes. The reduced BBB damage accompanies decreased neutrophil infiltration and proinflammatory gene expression in the injured brain parenchyma, which may account for the attenuated brain damage in TLR2 KO mice after ICH. Conclusions: TLR2 plays a detrimental role in ICH-induced brain damage by activating MMP9 in astrocytes, compromising BBB, and enhancing neutrophils infiltration and proinflammatory gene expression. © 2015 Min et al.; licensee BioMed Central.
URI
http://hdl.handle.net/20.500.11750/2347
DOI
10.1186/s13041-015-0116-z
Publisher
BioMed Central Ltd.
Related Researcher
Files:
There are no files associated with this item.
Collection:
Brain and Cognitive SciencesETC1. Journal Articles


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