Cited 25 time in webofscience Cited 26 time in scopus

Trithorax Group Protein Oryza sativa Trithorax1 Controls Flowering Time in Rice via Interaction with Early heading date3

Title
Trithorax Group Protein Oryza sativa Trithorax1 Controls Flowering Time in Rice via Interaction with Early heading date3
Authors
Choi, SC[Choi, Sang Chul]Lee, S[Lee, Shinyoung]Kim, SR[Kim, Sung-Ryul]Lee, YS[Lee, Yang-Seok]Liu, CY[Liu, Chunyan]Cao, XF[Cao, Xiaofeng]An, G[An, Gynheung]
DGIST Authors
Lee, S[Lee, Shinyoung]
Issue Date
2014-03
Citation
Plant Physiology, 164(3), 1326-1337
Type
Article
Article Type
Article
Keywords
Amino Acid MotifsAmino Acid SequenceBacterial DNABiological ModelChemistryChromosome SegregationDNA, BacterialFlowerFlowersGene Expression RegulationGene Expression Regulation, PlantGenes, PlantGeneticsHistoneHistonesImmunoprecipitationMessenger RNAMetabolismMethylationModels, BiologicalMolecular GeneticsMolecular Sequence DataMutationNucleosomeNucleosomesOryza SativaPhenotypePhotoperiodPhotoperiodicityPhysiologyPlant GenePlant ProteinsPlants, Genetically ModifiedProtein BindingProtein MotifProtein Structure, TertiaryProtein Tertiary StructureReal-Time Polymerase Chain ReactionReal Time Polymerase Chain ReactionRecombinant ProteinRecombinant ProteinsRiceRNA InterferenceRNA, MessengerT-DNAT DNATimeTime FactorsTransgenic PlantVegetable Protein
ISSN
0032-0889
Abstract
Trithorax group proteins are chromatin-remodeling factors that activate target gene expression by antagonistically functioning against the Polycomb group. In Arabidopsis (Arabidopsis thaliana), Arabidopsis Trithorax protein1 (ATX1) regulates flowering time and floral organ identity. Here, we observed that suppression of Oryza sativa Trithorax1 (OsTrx1), an ortholog of ATX1, delayed flowering time in rice (Oryza sativa). Because the delay occurred only under long-day conditions, we evaluated the flowering signal pathways that specifically function under long-day conditions. Among them, the OsMADS50 and Heading date1 pathways were not affected by the mutation. However, the Grain number, plant height, and heading date7 (Ghd7) pathway was altered in ostrx1. Transcript levels of OsGI, phytochrome genes, and Early heading date3 (Ehd3), which function upstream of Ghd7, were unchanged in the mutant. Because Trx group proteins form a complex with other proteins to modify the chromatin structure of target genes, we investigated whether OsTrx1 interacts with a previously identified protein that functions upstream of Ghd7. We demonstrated that the plant homeodomain motif of OsTrx1 binds to native histone H3 from the calf thymus and that OsTrx1 binds to Ehd3 through the region between the plant homeodomain and SET domains. Finally, we showed that the SET domain at the C-terminal end of OsTrx1 has histone H3 methyltransferase activity when incubated with oligonucleosomes. Our results suggest that OsTrx1 plays an important role in regulating flowering time in rice by modulating chromatin structure. © 2014 American Society of Plant Biologists. All rights reserved.
URI
http://hdl.handle.net/20.500.11750/2396
DOI
10.1104/pp.113.228049
Publisher
American Society of Plant Biologists
Files:
There are no files associated with this item.
Collection:
ETC1. Journal Articles


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