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The Arabidopsis NAC Transcription Factor ANAC096 Cooperates with bZIP-Type Transcription Factors in Dehydration and Osmotic Stress Responses

The Arabidopsis NAC Transcription Factor ANAC096 Cooperates with bZIP-Type Transcription Factors in Dehydration and Osmotic Stress Responses
Xu, ZY[Xu, Zheng-Yi]Kim, SY[Kim, Soo Youn]Hyeon, DY[Hyeon, Do Young]Kim, DH[Kim, Dae Heon]Dong, T[Dong, Ting]Park, Y[Park, Youngmin]Jin, JB[Jin, Jing Bo]Joo, SH[Joo, Se-Hwan]Kim, SK[Kim, Seong-Ki]Hong, JC[Hong, Jong Chan]Hwang, D[Hwang, Daehee]Hwang, I[Hwang, Inhwan]
DGIST Authors
Hwang, D[Hwang, Daehee]
Issue Date
Plant Cell, 25(11), 4708-4724
Article Type
ABF2 Protein, ArabidopsisABF3 Protein, ArabidopsisAbscisic Acid (ABA)ArabidopsisArabidopsis ProteinArabidopsis ProteinsBase SequenceBasic-Leucine Zipper Transcription FactorBasic-Leucine Zipper Transcription FactorsCell NucleusDehydrationDNA-Binding ProteinDNA-Binding ProteinsDrug EffectGene Expression RegulationGene Expression Regulation, PlantGeneticsGerminationGrowth, Development and AgingMetabolismMolecular GeneticsMolecular Sequence DataMutationNucleotide SequenceOsmotic PressurePhysiological StressPhysiologyPlants, Genetically ModifiedPromoter RegionPromoter Regions, GeneticRD29a Protein, ArabidopsisStress, PhysiologicalTranscription FactorTranscription FactorsTransgenic Plant
Multiple transcription factors (TFs) play essential roles in plants under abiotic stress, but how these multiple TFs cooperate in abiotic stress responses remains largely unknown. In this study, we provide evidence that the NAC (for NAM, ATAF1/2, and CUC2) TF ANAC096 cooperates with the bZIP-type TFs ABRE binding factor and ABRE binding protein (ABF/AREB) to help plants survive under dehydration and osmotic stress conditions. ANAC096 directly interacts with ABF2 and ABF4, but not with ABF3, both in vitro and in vivo. ANAC096 and ABF2 synergistically activate RD29A transcription. Our genome-wide gene expression analysis revealed that a major proportion of abscisic acid (ABA)-responsive genes are under the transcriptional regulation of ANAC096. We found that the Arabidopsis thaliana anac096 mutant is hyposensitive to exogenous ABA and shows impaired ABA-induced stomatal closure and increased water loss under dehydration stress conditions. Furthermore, we found the anac096 abf2 abf4 triple mutant is much more sensitive to dehydration and osmotic stresses than the anac096 single mutant or the abf2 abf4 double mutant. Based on these results, we propose that ANAC096 is involved in a synergistic relationship with a subset of ABFs for the transcriptional activation of ABA-inducible genes in response to dehydration and osmotic stresses. © 2013 American Society of Plant Biologists. All rights reserved.
American Society of Plant Biologists
Related Researcher
  • Author Hwang, Dae Hee Systems Biology and Medicine Lab
  • Research Interests Multilayered spatiotemporal networks; Regulatory motifs or pathways; Metabolite-protein networks; Network stochasticity; Proteomics and informatics
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Department of New BiologySystems Biology and Medicine Lab1. Journal Articles

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