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Ultrastructural Analysis of the Synaptic Connectivity of TRPV1-Expressing Primary Afferent Terminals in the Rat Trigeminal Caudal Nucleus

Title
Ultrastructural Analysis of the Synaptic Connectivity of TRPV1-Expressing Primary Afferent Terminals in the Rat Trigeminal Caudal Nucleus
Authors
Yeo, EJ[Yeo, Eun Jin]Cho, YS[Cho, Yi Sul]Paik, SK[Paik, Sang Kyoo]Yoshida, A[Yoshida, Atsushi]Park, MJ[Park, Mae Ja]Ahn, DK[Ahn, Dong Kuk]Moon, C[Moon, Cheil]Kim, YS[Kim, Yun Sook]Bae, YC[Bae, Yong Chul]
DGIST Authors
Moon, C[Moon, Cheil]
Issue Date
2010-10-15
Citation
Journal of Comparative Neurology, 518(20), 4134-4146
Type
Article
Article Type
Article
Keywords
4 Aminobutyric AcidAnimal TissueAnimalsAntibody LabelingCell UltrastructureCell VacuoleDendriteDendritic SpineElectron MicroscopyEnkephalinGamma-Aminobutyric AcidGlutamate DecarboxylaseGlutamate Decarboxylase 65Glutamate Decarboxylase 67ImmunohistochemistryMaleMicroscopy, ImmunoelectronMyelinated NerveNerve FiberNeurons, AfferentNociceptionNon-HumanNon-Myelinated NervePresynaptic NervePresynaptic TerminalsPriority JournalProtein ExpressionRatRatsRats, Sprague-DawleySynapseSynapsesSynaptic MembraneTrigeminalTrigeminal Caudal NucleusTrigeminal NerveTrigeminal NucleusTRPV Cation ChannelsTRPV1Vanilloid Receptor1
ISSN
0021-9967
Abstract
Trigeminal primary afferents that express the transient receptor potential vanilloid 1 (TRPV1) are important for the transmission of orofacial nociception. However, little is known about how the TRPV1-mediated nociceptive information is processed at the first relay nucleus in the central nervous system (CNS). To address this issue, we studied the synaptic connectivity of TRPV1-positive (+) terminals in the rat trigeminal caudal nucleus (Vc) by using electron microscopic immunohistochemistry and analysis of serial thin sections. Whereas the large majority of TRPV1+ terminals made synaptic contacts of an asymmetric type with one or two postsynaptic dendrites, a considerable fraction also participated in complex glomerular synaptic arrangements. A few TRPV1+ terminals received axoaxonic contacts from synaptic endings that contained pleomorphic synaptic vesicles and were immunolabeled for glutamic acid decarboxylase, the synthesizing enzyme for the inhibitory neurotransmitter c-aminobutyric acid (GABA). We classified the TRPV1+ terminals into an S-type, containing less than five dense-core vesicles (DCVs), and a DCV-type, containing five or more DCVs. The number of postsynaptic dendrites was similar between the two types of terminals; however, whereas axoaxonic contacts were frequent on the S-type, the DCV-type did not receive axoaxonic contacts. In the sensory root of the trigeminal ganglion, TRPV1+ axons were mostly unmyelinated, and a small fraction was small myelinated. These results suggest that the TRPV1-mediated nociceptive information from the orofacial region is processed in a specific manner by two distinct types of synaptic arrangements in the Vc, and that the central input of a few TRPV1+ afferents is presynaptically modulated via a GABA-mediated mechanism. © 2010 Wiley-Liss, Inc.
URI
http://hdl.handle.net/20.500.11750/2491
DOI
10.1002/cne.22369
Publisher
Wiley
Related Researcher
Files:
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Collection:
Brain and Cognitive SciencesETC1. Journal Articles


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