Cited 0 time in webofscience Cited 0 time in scopus

A role for Toll-like receptor 4 in the host response to the lung infection of Yersinia pseudotuberculosis in mice

Title
A role for Toll-like receptor 4 in the host response to the lung infection of Yersinia pseudotuberculosis in mice
Authors
Choi, JA[Choi, Jin-A]Jeong, YJ[Jeong, Yu-Jin]Kim, JE[Kim, Jae-Eun]Kang, MJ[Kang, Min-Jung]Kim, JC[Kim, Jee-Cheon]Oh, SM[Oh, Sang-Muk]Lee, KB[Lee, Kyung-Bok]Kim, DH[Kim, Dong-Hyun]Kim, DJ[Kim, Dong-Jae]Park, JH[Park, Jong-Hwan]
DGIST Authors
Kim, DJ[Kim, Dong-Jae]
Issue Date
2016-02
Citation
Comparative Immunology Microbiology and Infectious Diseases, 44, 54-60
Type
Article
Article Type
Article
Keywords
Animal CellAnimal ExperimentAnimal ModelAnimal TissueBacterial ColonizationBacterial StrainBacterial TransmissionBlood SamplingBloodstream InfectionControlled StudyCXCL2 ChemokineDisease SeverityHistopathologyImmune ResponseInfection PreventionInfectious HepatitisLung InfectionMouseNon-HumanPathogenesisProtein DepletionProtein FunctionSpleen DiseaseSurvival RateSystemic DisseminationToll-Like Receptor4Tumor Necrosis Factor-AlphaYersinia PseudotuberculosisYersinia Pseudotuberculosis Infection
ISSN
0147-9571
Abstract
Although a Yersinia pseudotuberculosis (Yptb) lung infection model has been developed to study Y. pestis pathogenesis, it is still necessary to establish a new animal model to mimic the pathophysiological features induced by Y. pestis infection. Here, we provide a new lung infection model using the Yptb strain, IP2777, which displayed rapid spread of bacteria to the liver, spleen, and blood. In addition, we examined whether TLR4 is involved in Yptb-induced pathogenesis in the lung infection model of mice we generated. Following lung infection of WT and TLR4-deficient mice with the Yptb strain IP2777, the survival rate, bacterial colonization, histopathology, and level of cytokines and chemokines in the lung, spleen, liver, and blood were analyzed. TLR4-deficient mice had a lower survival rate than WT mice in response to Yptb lung infection. Although the bacterial colonization and pathology of the lung were comparable between WT and TLR4-deficient mice, those of the spleen and liver were more severe in TLR4-deficient mice. In addition, the levels of TNF-α and CXCL2 in the liver and IL-and CXCL2 in the blood were higher in TLR4-deficient mice than in WT mice. Our results demonstrate that TLR4 is necessary for optimal host protection against Yptb lung infection and TLR4-deficient mice may serve as a better genetic model of Yptb infection for mimicking Y. pestis infection. © 2016 Elsevier Ltd.
URI
http://hdl.handle.net/20.500.11750/2552
DOI
10.1016/j.cimid.2016.01.001
Publisher
Elsevier Ltd
Files:
There are no files associated with this item.
Collection:
Laboratory Animal Resource Center1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE