Cited 21 time in webofscience Cited 26 time in scopus

ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging

Title
ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging
Authors
Lee, E[Lee, Eunsoo]Choi, J[Choi, Jungyoon]Jo, Y[Jo, Youhwa]Kim, JY[Kim, Joo Yeon]Jang, YJ[Jang, Yu Jin]Lee, HM[Lee, Hye Myeong]Kim, SY[Kim, So Yeun]Lee, HJ[Lee, Ho-Jae]Cho, K[Cho, Keunchang]Jung, N[Jung, Neoncheol]Hur, EM[Hur, Eun Mi]Jeong, SJ[Jeong, Sung Jin]Moon, C[Moon, Cheil]Choe, Y[Choe, Youngshik]Rhyu, IJ[Rhyu, Im Joo]Kim, H[Kim, Hyun]Sun, W[Sun, Woong]
DGIST Authors
Moon, C[Moon, Cheil]
Issue Date
2016-01-11
Citation
Scientific Reports, 6
Type
Article
Article Type
Article
ISSN
2045-2322
Abstract
Understanding the structural organization of organs and organisms at the cellular level is a fundamental challenge in biology. This task has been approached by reconstructing three-dimensional structure from images taken from serially sectioned tissues, which is not only labor-intensive and time-consuming but also error-prone. Recent advances in tissue clearing techniques allow visualization of cellular structures and neural networks inside of unsectioned whole tissues or the entire body. However, currently available protocols require long process times. Here, we present the rapid and highly reproducible ACT-PRESTO (active clarity technique-pressure related efficient and stable transfer of macromolecules into organs) method that clears tissues or the whole body within 1 day while preserving tissue architecture and protein-based signals derived from endogenous fluorescent proteins. Moreover, ACT-PRESTO is compatible with conventional immunolabeling methods and expedites antibody penetration into thick specimens by applying pressure. The speed and consistency of this method will allow high-content mapping and analysis of normal and pathological features in intact organs and bodies.
URI
http://hdl.handle.net/20.500.11750/2556
DOI
10.1038/srep18631
Publisher
Nature Publishing Group
Related Researcher
Files:
There are no files associated with this item.
Collection:
Brain and Cognitive SciencesETC1. Journal Articles


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