Cited 2 time in webofscience Cited 3 time in scopus

Arabidopsis VIM Proteins Regulate Epigenetic Silencing by Modulating DNA Methylation and Histone Modification in Cooperation with MET1

Title
Arabidopsis VIM Proteins Regulate Epigenetic Silencing by Modulating DNA Methylation and Histone Modification in Cooperation with MET1
Authors
Kim, JeongsikKim, Jin HeeRichards, Eric J.Chung, Kyung MinWoo, Hye Ryun
DGIST Authors
Kim, Jeongsik; Kim, Jin Hee; Woo, Hye Ryun
Issue Date
2014-09
Citation
Molecular Plant, 7(9), 1470-1485
Type
Article
Article Type
Article
Keywords
AnimaliaArabidopsisArabidopsis ProteinArabidopsis ProteinsDNA (Cytosine-5-)-MethyltransferaseDNA MethylationEpigenetic SilencingGene LocusGene SilencingGenetic LociGenetic TranscriptionGeneticsGenome, PlantHeterochromatinHistoneHistone ModificationHistonesMET1MET1 Protein, ArabidopsisMetabolismMutationPlant GenomePromoter RegionPromoter Regions, GeneticSRATranscription, GeneticVIM/ORTH
ISSN
1674-2052
Abstract
Methylcytosine-binding proteins containing SRA (SET- and RING-Associated) domain are required for the establishment and/or maintenance of DNA methylation in both plants and animals. We previously proposed that Arabidopsis VIM/ORTH proteins with an SRA domain maintain DNA methylation and epigenetic gene silencing in heterochromatic regions. However, their endogenous targets of epigenetic gene silencing have not been analyzed globally and the mechanisms by which VIM proteins coordinate DNA methylation and epigenetic silencing are largely unknown. In this study, a genome-wide transcript profiling analysis revealed 544 derepressed genes in a vim1/2/3 triple mutant, including 133 known genes. VIM1 bound to promoter and transcribed regions of the up-regulated genes in vim1/2/3 and VIM deficiency caused severe DNA hypomethylation in all sequence contexts at direct VIM1 targets. We found a drastic loss of H3K9me2 at heterochromatic chromocenters in vim1/2/3 nuclei. Furthermore, aberrant changes in transcriptionally active and repressive histone modifications were observed at VIM1 targets in vim1/2/3. VIM1-binding capacity to target genes was significantly reduced in the met1 background, indicating that VIM1 primarily recognizes CG methylation deposited by MET1. Overall, our data indicate that VIM proteins regulate genome-wide epigenetic gene silencing through coordinated modulation of DNA methylation and histone modification status in collaboration with MET1. © 2014 The Authors. All rights reserved.
URI
http://hdl.handle.net/20.500.11750/2646
DOI
10.1093/mp/ssu079
Publisher
Cell Press
Related Researcher
Files:
There are no files associated with this item.
Collection:
New BiologyCBRG(Complex Biology Research Group)1. Journal Articles
New BiologyETC1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE