Cited 56 time in webofscience Cited 59 time in scopus

Border Control-A Membrane-Linked Interactome of Arabidopsis

Title
Border Control-A Membrane-Linked Interactome of Arabidopsis
Authors
Jones, AM[Jones, Alexander M.]Xuan, YH[Xuan, Yuanhu]Xu, M[Xu, Meng]Wang, RS[Wang, Rui-Sheng]Ho, CH[Ho, Cheng-Hsun]Lalonde, S[Lalonde, Sylvie]You, CH[You, Chang Hun]Sardi, MI[Sardi, Maria I.]Parsa, SA[Parsa, Saman A.]Smith-Valle, E[Smith-Valle, Erika]Su, TY[Su, Tianying]Frazer, KA[Frazer, Keith A.]Pilot, G[Pilot, Guillaume]Pratelli, R[Pratelli, Rejane]Grossmann, G[Grossmann, Guido]Acharya, BR[Acharya, Biswa R.]Hu, HC[Hu, Heng-Cheng]Engineer, C[Engineer, Cawas]Villiers, F[Villiers, Florent]Ju, CL[Ju, Chuanli]Takeda, K[Takeda, Kouji]Su, Z[Su, Zhao]Dong, QF[Dong, Qunfeng]Assmann, SM[Assmann, Sarah M.]Chen, J[Chen, Jin]Kwak, JM[Kwak, June M.]Schroeder, JI[Schroeder, Julian I.]Albert, R[Albert, Reka]Rhee, SY[Rhee, Seung Y.]Frommer, WB[Frommer, Wolf B.]
DGIST Authors
Kwak, JM[Kwak, June M.]
Issue Date
2014-05-16
Citation
Science, 344(6185), 711-716
Type
Article
Article Type
Article
Keywords
Abscisic Acid (ABA)AngiospermAquaporinArabidopsisArabidopsis ProteinArabidopsis ProteinsBiological Phenomena and Functions Concerning the Entire OrganismBrassinolideBrassinosteroidCell MembraneEnzyme ActivityFunctional GenomicsGene ExpressionGeneticsGreen Fluorescent ProteinGuanine Nucleotide Binding ProteinHeterotrimeric Guanine Nucleotide Binding ProteinHormoneMembraneMembrane ProteinMembrane ProteinsMembrane ReceptorMetabolismNon-HumanOligomerizationPlantPotassium ChannelPriority JournalProteinProtein FamilyProtein InteractionProtein Interaction MapsProtein Protein InteractionProtein Serine Threonine KinaseProtein SubunitSignal TransductionSignalingSolute TransportSpaceTwo-Hybrid SystemTwo-Hybrid System TechniquesUbiquitinYeast
ISSN
0036-8075
Abstract
Cellular membranes act as signaling platforms and control solute transport. Membrane receptors, transporters, and enzymes communicate with intracellular processes through protein-protein interactions. Using a split-ubiquitin yeast two-hybrid screen that covers a test-space of 6.4 × 106 pairs, we identified 12,102 membrane/signaling protein interactions from Arabidopsis. Besides confirmation of expected interactions such as heterotrimeric G protein subunit interactions and aquaporin oligomerization, >99% of the interactions were previously unknown. Interactions were confirmed at a rate of 32% in orthogonal in planta split-green flourescent protein interaction assays, which was statistically indistinguishable from the confirmation rate for known interactions collected from literature (38%). Regulatory associations in membrane protein trafficking, turnover, and phosphorylation include regulation of potassium channel activity through abscisic acid signaling, transporter activity by a WNK kinase, and a brassinolide receptor kinase by trafficking-related proteins. These examples underscore the utility of the membrane/signaling protein interaction network for gene discovery and hypothesis generation in plants and other organisms.
URI
http://hdl.handle.net/20.500.11750/2653
DOI
10.1126/science.1251358
Publisher
American Association for the Advancement of Science
Related Researcher
  • Author Kwak, June Myoung KWAK_LAB(Lab of Cell Signailng)
  • Research Interests Calcium Signaling; ABA;ROS Signaling; drought-tolerant plants; Development of Abscission; Culluar Features; Signaling network; Environmental Regulation
Files:
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Collection:
New BiologyETC1. Journal Articles


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