Cited 26 time in webofscience Cited 29 time in scopus

Anti-inflammatory effects of cordycepin in lipopolysaccharide-stimulated RAW 264.7 macrophages through Toll-like receptor 4-mediated suppression of mitogen-activated protein kinases and NF-kappa B signaling pathways

Title
Anti-inflammatory effects of cordycepin in lipopolysaccharide-stimulated RAW 264.7 macrophages through Toll-like receptor 4-mediated suppression of mitogen-activated protein kinases and NF-kappa B signaling pathways
Authors
Choi, YH[Choi, Yung Hyun]Kim, GY[Kim, Gi-Young]Lee, HH[Lee, Hye Hyeon]
DGIST Authors
Lee, HH[Lee, Hye Hyeon]
Issue Date
2014
Citation
Drug Design Development and Therapy, 8, 1941-1953
Type
Article
Article Type
Article
Keywords
AnimalAnimal CellAnimalsAntagonists and InhibitorsAnti-InflammationAnti-Inflammatory ActivityAnti-Inflammatory Agents, Non-SteroidalCell CultureCell Nucleus TransplantationCells, CulturedChemistryControlled StudyCordycepinCyclooxygenase 2Cytokine ReleaseDeoxyadenosine DerivativeDeoxyadenosinesDose-ResponseDose-Response Relationship, DrugDrug EffectDrug EffectsDrug MechanismEnzyme InactivationEnzyme InhibitionEnzyme PhosphorylationEnzyme RepressionFatty Acid SynthesisGene Expression RegulationImmunoglobulin Enhancer Binding ProteinInducible Nitric Oxide SynthaseInterleukin-1 BetaLipopolysaccharideLipopolysaccharidesMacrophageMacrophagesMetabolismMiceMitogen-Activated Protein KinaseMitogen-Activated Protein KinasesMouseNF-Kappa BNitric OxideNon-HumanNon-Steroid Antiinflammatory AgentOxidation-Reduction PotentialProstaglandin E2Protein BindingProtein Carbohydrate InteractionProtein DegradationProtein PhosphorylationProtein Synthesis InhibitionRaw 264.7 MacrophageSignal TransductionStructure-Activity RelationshipStructure Activity RelationSynaptotagmin IToll-Like Receptor4Tumor Necrosis Factor-Alpha
ISSN
1177-8881
Abstract
Cordycepin is the main functional component of the Cordyceps species, which has been widely used in traditional Oriental medicine. This compound possesses many pharmacological properties, such as an ability to enhance immune function, as well as antioxidant, antiaging, and anticancer effects. In the present study, we investigated the anti-inflammatory effects of cordycepin using a murine macrophage RAW 264.7 cell model. Our data demonstrated that cordycepin suppressed production of proinflammatory mediators such as nitric oxide (NO) and prostaglandin E2 by inhibiting inducible NO synthase and cyclooxygenase-2 gene expression. Cordycepin also inhibited the release of proinflammatory cytokines, including tumor necrosis factor-alpha and interleukin-1-beta, through downregulation of respective mRNA expression. In addition, pretreatment with cordycepin significantly inhibited lipopolysaccharide (LPS)-induced phosphorylation of mitogen-activating protein kinases and attenuated nuclear translocation of NF-κB by LPS, which was associated with abrogation of inhibitor kappa B-alpha degradation. Furthermore, cordycepin potently inhibited the binding of LPS to macrophages and LPS-induced Toll-like receptor 4 and myeloid differentiation factor 88 expression. Taken together, the results suggest that the inhibitory effects of cordycepin on LPS-stimulated inflammatory responses in RAW 264.7 macrophages are associated with suppression of mitogen-activating protein kinases and activation of NF-κB by inhibition of the Toll-like receptor 4 signaling pathway. © 2014 Choi et al.
URI
http://hdl.handle.net/20.500.11750/2681
DOI
10.2147/DDDT.S71957
Publisher
Dove Medical Press Ltd.
Files:
There are no files associated with this item.
Collection:
New BiologyETC1. Journal Articles


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