Cited 14 time in webofscience Cited 14 time in scopus

Electrochemical biosensor for Mycobacterium tuberculosis DNA detection based on gold nanotubes array electrode platform

Title
Electrochemical biosensor for Mycobacterium tuberculosis DNA detection based on gold nanotubes array electrode platform
Authors
Torati, R[Torati, Ramulu]Reddy, V[Reddy, Venu]Yoon, SS[Yoon, Seok Soo]Kim, C[Kim, CheolGi]
DGIST Authors
Torati, R[Torati, Ramulu]; Reddy, V[Reddy, Venu]; Kim, C[Kim, CheolGi]
Issue Date
2016-04-15
Citation
Biosensors and Bioelectronics, 78, 483-488
Type
Article
Article Type
Article
Keywords
Aromatic CompoundsBacterial DNABiosensing TechniquesBiosensorBiosensorsChemistryComplementary DnaCyclic PotentiometryCyclic VoltammetryDNADNA BiosensorDNA BiosensorsDNA DeterminationDNA HybridizationDNA ProbeDNA, BacterialElectro-Chemical ElectrodesElectrochemical AnalysisElectrochemical BiosensorElectrochemical DepositionElectrochemical DNA BiosensorsElectrochemical PerformanceElectrochemical TechniquesElectrodepositionElectrodesElectron TransportGenetic ProceduresGoldGold NanotubeGold Nanotube ArrayHumanHumansHybridizationIsolation and PurificationLimit of DetectionMethylene BlueMicrobiologyMicroscopy, Electron, ScanningMycobacterium TuberculosisNanoarrayNanofabricationNanotubeNanotubesNon-HumanPathogenicityReductionScanning Electron MicroscopySynthesis (Chemical)Thick FilmsTuberculosisUnclassified DrugYarn
ISSN
0956-5663
Abstract
The template assisted electrochemical deposition technique was used for the synthesis of gold nanotubes array (AuNTsA). The morphological structure of the synthesized AuNTsA was observed by scanning electron microscopy and found that the individual nanotubes are around 1.5μm in length with a diameter of 200nm. Nanotubes are vertically aligned to the Au thick film, which is formed during the synthesis process of nanotubes. The electrochemical performance of the AuNTsA was compared with the bare Au electrode and found that AuNTsA has better electron transfer surface than bare Au electrode which is due to the high surface area. Hence, the AuNTsA was used as an electrode for the fabrication of DNA hybridization biosensor for detection of Mycobacterium Tuberculosis DNA. The DNA hybridization biosensor constructed by AuNTsA electrode was characterized by cyclic voltammetry technique with Fe(CN)63-/4- as an electrochemical redox indicator. The selectivity of the fabricated biosensor was illustrated by hybridization with complementary DNA and non-complementary DNA with probe DNA immobilized AuNTsA electrode using methylene blue as a hybridization indicator. The developed electrochemical DNA biosensor shows good linear range of complementary DNA concentration from 0.01ng/μL to 100ng/μL with high detection limit. © 2015 Elsevier B.V.
URI
http://hdl.handle.net/20.500.11750/2695
DOI
10.1016/j.bios.2015.11.098
Publisher
Elsevier
Files:
There are no files associated with this item.
Collection:
Emerging Materials ScienceLab for NanoBio-Materials & SpinTronics(nBEST)1. Journal Articles


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