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Establishment of a mouse model for pulmonary inflammation and fibrosis by intratracheal instillation of polyhexamethyleneguanidine phosphate

Title
Establishment of a mouse model for pulmonary inflammation and fibrosis by intratracheal instillation of polyhexamethyleneguanidine phosphate
Authors
Lee, SJ[Lee, Sang Jin]Park, JH[Park, Jong-Hwan]Lee, JY[Lee, Jun-Young]Jeong, YJ[Jeong, Yu-Jin]Song, JA[Song, Jeong Ah]Lee, K[Lee, Kyuhong]Kim, DJ[Kim, Dong-Jae]
DGIST Authors
Kim, DJ[Kim, Dong-Jae]
Issue Date
2016
Citation
Journal of Toxicologic Pathology, 29(2), 95-102
Type
Article
Article Type
Article
Keywords
Animal ExperimentAnimal ModelAnimal TissueControlled StudyCXCL1 ChemokineCytokine ProductionDisease SeverityFemaleHistopathologyInterleukin-6Lung FibrosisLung InfiltrateLung InflammationLung ParenchymaMiceMonocyte Chemotactic Protein 1Mononuclear CellMouseMurine ModelNeutrophilNon-HumanPhosphatePneumoniaPolyhexamethyleneguanidine PhosphatePulmonary FibrosisTransforming Growth Factor BetaTumor Necrosis Factor-AlphaUnclassified DrugWeight Change
ISSN
0914-9198
Abstract
Although several animal models have been developed to study human pulmonary fibrosis, lack of a perfect model has raised the need for various animal models of pulmonary fibrosis. In this study, we evaluated the pulmonary effect of polyhexamethyleneguanidine phosphate instillation into the lungs of mice to determine the potential of these mice as a murine model of pulmonary fibrosis. Intratracheal instillation of polyhexamethyleneguanidine phosphate induced severe lung inflammation manifested by the infiltration of mononuclear cells and neutrophils and increased production of IL-6, TNF-α, CCL2 and CXCL1. The lung inflammation gradually increased until 28 days after polyhexamethyleneguanidine phosphate exposure, and increases of collagen deposition and TGF-β production, which are indicators of pulmonary fibrosis, were seen. Our study showed that intratracheal instillation of polyhexamethyleneguanidine phosphate induces pulmonary inflammation and fibrosis in mice. © 2016 The Japanese Society of Toxicologic Pathology.
URI
http://hdl.handle.net/20.500.11750/2769
DOI
10.1293/tox.2015-0067
Publisher
Japanese Society of Toxicologic Pathology
Files:
There are no files associated with this item.
Collection:
Laboratory Animal Resource Center1. Journal Articles


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