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PGC-Enriched miRNAs Control Germ Cell Development
- PGC-Enriched miRNAs Control Germ Cell Development
- Bhin, J[Bhin, Jinhyuk]; Jeong, HS[Jeong, Hoe-Su]; Kim, JS[Kim, Jong Soo]; Shin, JO[Shin, Jeong Oh]; Hong, KS[Hong, Ki Sung]; Jung, HS[Jung, Han-Sung]; Kim, C[Kim, Changhoon]; Hwang, D[Hwang, Daehee]; Kim, KS[Kim, Kye-Seong]
- DGIST Authors
- Hwang, D[Hwang, Daehee]
- Issue Date
- Molecules and Cells, 38(10), 895-903
- Article Type
- Animal Tissue; Apoptosis; Bioinformatic Analysis; Cell Adhesion; Cell Cycle; Cell Differentiation; Cell Proliferation; Cell Transport; Cellular Distribution; Chromosome 1; Comparative Study; Controlled Study; Gene Expression; Gene Expression Profiling; In Situ Hybridization; Intracellular Signaling; Male; Male Primordial Germ Cells; Microarray; Microarray Analysis; MicroRNA; MicroRNA-106B; MicroRNA-10B; MicroRNA-126 3P; MicroRNA-127; MicroRNA-18A; MicroRNA-18B; MicroRNA-301; MicroRNA-93; MiRNAs; Mouse; Non-Human; Northern Blotting; Polymerase Chain Reaction; Prediction; Primordial Germ Cell; Protein Degradation; Reverse Transcriptase-Polymerase Chain Reaction; RNA Processing; Transcription Regulation; Unclassified Drug
- Non-coding microRNAs (miRNAs) regulate the translation of target messenger RNAs (mRNAs) involved in the growth and development of a variety of cells, including primordial germ cells (PGCs) which play an essential role in germ cell development. However, the target mRNAs and the regulatory networks influenced by miRNAs in PGCs remain unc lear. Here, we demonstrate a novel miRNAs control PGC development through targeting mRNAs involved in various cellular pathways. We reveal the PGC-enriched expression patterns of nine miRNAs, including miR-10b, -18a, -93, - 106b, -126-3p, -127, -181a, -181b, and -301, using miRNA expression analysis along with mRNA microarray analysi sin PGCs, embryonic gonads, and postnatal testes. These miRNAs are highly expressed in PGCs, as demonstrated by Northern blotting, miRNA in situ hybridization assay, and miRNA qPCR analysis. This integrative study utilizing mRNA microarray analysis and miRNA target prediction demonstrates the regulatory networks through whic h these miRNAs regulate their potential target genes during PGC development. The elucidated networks of miRNAs disclose a coordinated molecular mechanism by which these miRNAs regulate distinct cellular pathways in PGCs that determine germ cell development. © The Korean Society for Molecular and Cellular Biology. All rights reserved.
- Korean Society for Molecular and Cellular Biology
- Related Researcher
Hwang, Dae Hee
Systems Biology and Medicine Lab
Multilayered spatiotemporal networks; Regulatory motifs or pathways; Metabolite-protein networks; Network stochasticity; Proteomics and informatics
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