Cited 5 time in webofscience Cited 6 time in scopus

PGC-Enriched miRNAs Control Germ Cell Development

Title
PGC-Enriched miRNAs Control Germ Cell Development
Authors
Bhin, J[Bhin, Jinhyuk]Jeong, HS[Jeong, Hoe-Su]Kim, JS[Kim, Jong Soo]Shin, JO[Shin, Jeong Oh]Hong, KS[Hong, Ki Sung]Jung, HS[Jung, Han-Sung]Kim, C[Kim, Changhoon]Hwang, D[Hwang, Daehee]Kim, KS[Kim, Kye-Seong]
DGIST Authors
Hwang, D[Hwang, Daehee]
Issue Date
2015-10
Citation
Molecules and Cells, 38(10), 895-903
Type
Article
Article Type
Article
Keywords
Animal TissueApoptosisBioinformatic AnalysisCell AdhesionCell CycleCell DifferentiationCell ProliferationCell TransportCellular DistributionChromosome 1Comparative StudyControlled StudyGene ExpressionGene Expression ProfilingIn Situ HybridizationIntracellular SignalingMaleMale Primordial Germ CellsMicroarrayMicroarray AnalysisMicroRNAMicroRNA-106BMicroRNA-10BMicroRNA-126 3PMicroRNA-127MicroRNA-18AMicroRNA-18BMicroRNA-301MicroRNA-93MiRNAsMouseNon-HumanNorthern BlottingPolymerase Chain ReactionPredictionPrimordial Germ CellProtein DegradationReverse Transcriptase-Polymerase Chain ReactionRNA ProcessingTranscription RegulationUnclassified Drug
ISSN
1016-8478
Abstract
Non-coding microRNAs (miRNAs) regulate the translation of target messenger RNAs (mRNAs) involved in the growth and development of a variety of cells, including primordial germ cells (PGCs) which play an essential role in germ cell development. However, the target mRNAs and the regulatory networks influenced by miRNAs in PGCs remain unc lear. Here, we demonstrate a novel miRNAs control PGC development through targeting mRNAs involved in various cellular pathways. We reveal the PGC-enriched expression patterns of nine miRNAs, including miR-10b, -18a, -93, - 106b, -126-3p, -127, -181a, -181b, and -301, using miRNA expression analysis along with mRNA microarray analysi sin PGCs, embryonic gonads, and postnatal testes. These miRNAs are highly expressed in PGCs, as demonstrated by Northern blotting, miRNA in situ hybridization assay, and miRNA qPCR analysis. This integrative study utilizing mRNA microarray analysis and miRNA target prediction demonstrates the regulatory networks through whic h these miRNAs regulate their potential target genes during PGC development. The elucidated networks of miRNAs disclose a coordinated molecular mechanism by which these miRNAs regulate distinct cellular pathways in PGCs that determine germ cell development. © The Korean Society for Molecular and Cellular Biology. All rights reserved.
URI
http://hdl.handle.net/20.500.11750/2839
DOI
10.14348/molcells.2015.0146
Publisher
Korean Society for Molecular and Cellular Biology
Files:
There are no files associated with this item.
Collection:
New BiologySystems Biology and Medicine Lab1. Journal Articles


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