Cited 1 time in webofscience Cited 2 time in scopus

Mass propagation of microtubers from suspension cultures of Pinellia ternata cells and quantitative analysis of succinic acid in Pinellia tubers

Title
Mass propagation of microtubers from suspension cultures of Pinellia ternata cells and quantitative analysis of succinic acid in Pinellia tubers
Authors
Jie, EY[Jie, Eun Yee]Ryu, YB[Ryu, Young Bae]Choi, SA[Choi, Seung A.]Ahn, MS[Ahn, Myung Suk]Liu, JR[Liu, Jang Ryol]Min, SR[Min, Sung Ran]Kim, SW[Kim, Suk Weon]
DGIST Authors
Liu, JR[Liu, Jang Ryol]
Issue Date
2015-09
Citation
Plant Biotechnology Reports, 9(5), 331-338
Type
Article
Article Type
Article
Keywords
Microtuber ProductionPinellia TernataPlant RegenerationSuccinic Acid
ISSN
1863-5466
Abstract
The conditions for efficient tuber production from suspension cultures of Pinellia ternata cells which is one of medicinal herbs were established and succinic acid in tubers propagated in vitro was determined. Leaf explants formed white nodular structures and off-white calluses at a frequency of 90.6 % when cultured on Murashige and Skoog medium supplemented with 0.54 μM α-naphthaleneacetic acid (NAA); however, this frequency declined substantially with increasing NAA concentration, up to 16.2 μM, at which the frequency reached zero percent. In combination treatments with 4.44 μM 6-benzyladenine (BA) and NAA, however, the frequency of white nodular structure and off-white callus formation from leaf explants did not decrease, even at 16.2 μM NAA. Suspension cultures of P. ternata cells were established from leaf-derived off-white calluses in MS liquid medium containing 5.4 μM NAA and 4.44 μM BA. Upon plating onto MS basal medium, over 90 % of cell aggregates gave rise to microtubers and developed into plantlets. Regenerated plantlets were transplanted in potting soil and grown to maturity in a growth chamber, with a survival rate of >90 %. The highest succinic acid content in suspension culture-derived microtubers was 45 g/kg of extract. Compared with wild P. ternata medicinal tubers, the succinic acid content was very similar. The in vitro P. ternata microtuber proliferation system established in this study is thus an efficient alternative for the mass production of medicinal tubers. © 2015, Korean Society for Plant Biotechnology and Springer Japan.
URI
http://hdl.handle.net/20.500.11750/2857
DOI
10.1007/s11816-015-0369-0
Publisher
Springer
Files:
There are no files associated with this item.
Collection:
School of Undergraduate Studies1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE