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Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells

Title
Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells
Authors
Boo, K[Boo, Kyungjin]Bhin, J[Bhin, Jinhyuk]Jeon, Y[Jeon, Yoon]Kim, J[Kim, Joomyung]Shin, HJR[Shin, Hi-Jai R.]Park, JE[Park, Jong-Eun]Kim, K[Kim, Kyeongkyu]Kim, CR[Kim, Chang Rok]Jang, H[Jang, Hyonchol]Kim, IH[Kim, In-Hoo]Kim, VN[Kim, V. Narry]Hwang, D[Hwang, Daehee]Lee, H[Lee, Ho]Baek, SH[Baek, Sung Hee]
DGIST Authors
Hwang, D[Hwang, Daehee]
Issue Date
2015-04
Citation
Nature Communications, 6
Type
Article
Article Type
Article
ISSN
2041-1723
Abstract
The actions of transcription factors, chromatin modifiers and noncoding RNAs are crucial for the programming of cell states. Although the importance of various epigenetic machineries for controlling pluripotency of embryonic stem (ES) cells has been previously studied, how chromatin modifiers cooperate with specific transcription factors still remains largely elusive. Here, we find that Pontin chromatin remodelling factor plays an essential role as a coactivator for Oct4 for maintenance of pluripotency in mouse ES cells. Genome-wide analyses reveal that Pontin and Oct4 share a substantial set of target genes involved in ES cell maintenance. Intriguingly, we find that the Oct4-dependent coactivator function of Pontin extends to the transcription of large intergenic noncoding RNAs (lincRNAs) and in particular linc1253, a lineage programme repressing lincRNA, is a Pontin-dependent Oct4 target lincRNA. Together, our findings demonstrate that the Oct4-Pontin module plays critical roles in the regulation of genes involved in ES cell fate determination.
URI
http://hdl.handle.net/20.500.11750/2913
DOI
10.1038/ncomms7810
Publisher
Nature Publishing Group
Related Researcher
  • Author Hwang, Dae Hee Systems Biology and Medicine Lab
  • Research Interests Multilayered spatiotemporal networks; Regulatory motifs or pathways; Metabolite-protein networks; Network stochasticity; Proteomics and informatics
Files:
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Collection:
New BiologyETC1. Journal Articles


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