Cited 7 time in webofscience Cited 7 time in scopus

Diallyl trisulfide exerts anti-inflammatory effects in lipopolysaccharide-stimulated RAW 264.7 macrophages by suppressing the Toll-like receptor 4/nuclear factor-kappa B pathway

Title
Diallyl trisulfide exerts anti-inflammatory effects in lipopolysaccharide-stimulated RAW 264.7 macrophages by suppressing the Toll-like receptor 4/nuclear factor-kappa B pathway
Authors
Lee, HH[Lee, Hye Hyeon]Han, MH[Han, Min Ho]Hwang, HJ[Hwang, Hye Jin]Kim, GY[Kim, Gi-Young]Moon, SK[Moon, Sung-Kwon]Hyun, JW[Hyun, Jin-Won]Kim, WJ[Kim, Wun-Jae]Choi, YH[Choi, Yung Hyun]
DGIST Authors
Lee, HH[Lee, Hye Hyeon]
Issue Date
2015-02
Citation
International Journal of Molecular Medicine, 35(2), 487-495
Type
Article
Article Type
Article
Keywords
Animal CellAnimal ExperimentAnimal ModelAnti-Inflammatory ActivityAnti-Inflammatory AgentCli 095Controlled StudyCyclooxygenase 2CytotoxicityDiallyl TrisulfideDNA-BindingDrug MechanismDrug PotentiationEnzyme InhibitionGene ActivityGene RepressionGene TranslocationImmunoglobulin Enhancer Binding ProteinInducible Nitric Oxide SynthaseInflammationInterleukin-1 BetaIntracellular SignalingLipopolysaccharideMacrophageMyeloid Differentiation Factor 88Nitric OxideNon-Humannuclear factor-Kappa BProstaglandin E2Protein DegradationProtein ExpressionProtein InteractionProtein Serine Threonine Kinase InhibitorSynaptotagmin IToll-Like Receptor4Tumor Necrosis Factor-AlphaUnclassified Drug
ISSN
1107-3756
Abstract
Diallyl trisulfide (DATS; di-2-propen-1-yl trisulfide) is an organic polysulfide compound found in garlic and other allium vegetables. Although certain studies have demonstrated that DATS possesses strong anti-inflammatory activity, the underlying molecular mechanisms remain largely unresolved. In the present study, the anti-inflammatory potential of DATS was investigated using the murine macrophage RAW 264.7 cell model. At non-toxic concentrations, DATS inhibited the production of nitric oxide (NO) and prostaglandin E2 by inhibiting inducible NO synthase and cyclooxygenase-2 expression at the transcriptional level in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. DATS attenuated the release of the pro-inflammatory cytokines, tumor necrosis factor-α and interleukin-1β, by inhibiting mRNA expression, respectively. DATS also suppressed LPS-induced DNA-binding activity of nuclear factor-κB (NF-κB), as well as the nuclear translocation of the NF-κB p65, which correlated with the inhibitory effects of DATS on inhibitor-κB (IκB) degradation. In addition, DATS was observed to significantly suppress LPS-induced Toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 expression and the binding of LPS to macrophages, indicating the antagonistic effect of DATS against TLR4. Furthermore, blocking TLR4 signaling with the specific TLR4 signaling inhibitor, CLI-095, increased the anti-inflammatory potential of DATS in LPS-stimulated RAW 264.7 macrophages. These data demonstrate that DATS may attenuate the initiation of LPS-mediated intracellular signaling cascades by suppressing activation of NF-κB and by inhibiting binding of LPS to TLR4 on macrophages. © International Journal of Molecular Medicine 2014.
URI
http://hdl.handle.net/20.500.11750/2937
DOI
10.3892/ijmm.2014.2036
Publisher
Spandidos Publications
Files:
There are no files associated with this item.
Collection:
New BiologyETC1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE