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Diallyl trisulfide exerts anti-inflammatory effects in lipopolysaccharide-stimulated RAW 264.7 macrophages by suppressing the Toll-like receptor 4/nuclear factor-kappa B pathway
- Diallyl trisulfide exerts anti-inflammatory effects in lipopolysaccharide-stimulated RAW 264.7 macrophages by suppressing the Toll-like receptor 4/nuclear factor-kappa B pathway
- Lee, HH[Lee, Hye Hyeon]; Han, MH[Han, Min Ho]; Hwang, HJ[Hwang, Hye Jin]; Kim, GY[Kim, Gi-Young]; Moon, SK[Moon, Sung-Kwon]; Hyun, JW[Hyun, Jin-Won]; Kim, WJ[Kim, Wun-Jae]; Choi, YH[Choi, Yung Hyun]
- DGIST Authors
- Lee, HH[Lee, Hye Hyeon]
- Issue Date
- International Journal of Molecular Medicine, 35(2), 487-495
- Article Type
- Animal Cell; Animal Experiment; Animal Model; Anti-Inflammatory Activity; Anti-Inflammatory Agent; Cli 095; Controlled Study; Cyclooxygenase 2; Cytotoxicity; Diallyl Trisulfide; DNA-Binding; Drug Mechanism; Drug Potentiation; Enzyme Inhibition; Gene Activity; Gene Repression; Gene Translocation; Immunoglobulin Enhancer Binding Protein; Inducible Nitric Oxide Synthase; Inflammation; Interleukin-1 Beta; Intracellular Signaling; Lipopolysaccharide; Macrophage; Myeloid Differentiation Factor 88; Nitric Oxide; Non-Human; nuclear factor-Kappa B; Prostaglandin E2; Protein Degradation; Protein Expression; Protein Interaction; Protein Serine Threonine Kinase Inhibitor; Synaptotagmin I; Toll-Like Receptor4; Tumor Necrosis Factor-Alpha; Unclassified Drug
- Diallyl trisulfide (DATS; di-2-propen-1-yl trisulfide) is an organic polysulfide compound found in garlic and other allium vegetables. Although certain studies have demonstrated that DATS possesses strong anti-inflammatory activity, the underlying molecular mechanisms remain largely unresolved. In the present study, the anti-inflammatory potential of DATS was investigated using the murine macrophage RAW 264.7 cell model. At non-toxic concentrations, DATS inhibited the production of nitric oxide (NO) and prostaglandin E2 by inhibiting inducible NO synthase and cyclooxygenase-2 expression at the transcriptional level in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. DATS attenuated the release of the pro-inflammatory cytokines, tumor necrosis factor-α and interleukin-1β, by inhibiting mRNA expression, respectively. DATS also suppressed LPS-induced DNA-binding activity of nuclear factor-κB (NF-κB), as well as the nuclear translocation of the NF-κB p65, which correlated with the inhibitory effects of DATS on inhibitor-κB (IκB) degradation. In addition, DATS was observed to significantly suppress LPS-induced Toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 expression and the binding of LPS to macrophages, indicating the antagonistic effect of DATS against TLR4. Furthermore, blocking TLR4 signaling with the specific TLR4 signaling inhibitor, CLI-095, increased the anti-inflammatory potential of DATS in LPS-stimulated RAW 264.7 macrophages. These data demonstrate that DATS may attenuate the initiation of LPS-mediated intracellular signaling cascades by suppressing activation of NF-κB and by inhibiting binding of LPS to TLR4 on macrophages. © International Journal of Molecular Medicine 2014.
- Spandidos Publications
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