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Protein immobilization onto electrochemically synthesized CoFe nanowires

Title
Protein immobilization onto electrochemically synthesized CoFe nanowires
Authors
Torati, SR[Torati, Sri Ramulu]Reddy, V[Reddy, Venu]Yoon, SS[Yoon, Seok Soo]Kim, C[Kim, CheolGi]
DGIST Authors
Torati, SR[Torati, Sri Ramulu]; Reddy, V[Reddy, Venu]; Kim, C[Kim, CheolGi]
Issue Date
2015
Citation
International Journal of Nanomedicine, 10, 645-651
Type
Article
Article Type
Article
Keywords
3 AminopropyltriethoxysilaneBiofunctionalizationChemical CompositionCobalt Iron NanowireElectrodepositionN HydroxysuccinimideNanowirePhysical ChemistryPolycarbonateProtein ImmobilizationStreptavidinSynthesisUnclassified Drug
ISSN
1178-2013
Abstract
CoFe nanowires have been synthesized by the electrodeposition technique into the pores of a polycarbonate membrane with a nominal pore diameter of 50 nm, and the composition of CoFe nanowires varying by changing the source concentration of iron. The synthesized nanowire surfaces were functionalized with amine groups by treatment with aminopropyltriethoxysilane (APTES) linker, and then conjugated with streptavidin-Cy3 protein via ethyl (dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide coupling chemistry. The oxide surface of CoFe nanowire is easily modified with aminopropyltriethoxysilane to form an amine terminating group, which is covalently bonded to streptavidin-Cy3 protein. The physicochemical properties of the nanowires were analyzed through different characterization techniques such as scanning electron microscope, energy dispersive spectroscopy, and vibrating sample magnetometer. Fluorescence microscopic studies and Fourier transform infrared studies confirmed the immobilization of protein on the nanowire surface. In addition, the transmission electron microscope analysis reveals the thin protein layer which is around 12-15 nm on the nanowire surfaces. © 2015 Torati et al.
URI
http://hdl.handle.net/20.500.11750/2967
DOI
10.2147/IJN.S76850
Publisher
Dove Medical Press Ltd.
Files:
There are no files associated with this item.
Collection:
Emerging Materials ScienceLab for NanoBio-Materials & SpinTronics(nBEST)1. Journal Articles


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