Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Hwang, Jae Youn | - |
dc.contributor.author | Lim, Hae Gyun | - |
dc.contributor.author | Yoon, Chi Woo | - |
dc.contributor.author | Lam, Kwok Ho | - |
dc.contributor.author | Yoon, Sangpil | - |
dc.contributor.author | Lee, Changyang | - |
dc.contributor.author | Chiu, Chi Tat | - |
dc.contributor.author | Kang, Bong Jin | - |
dc.contributor.author | Kim, Hyung Ham | - |
dc.contributor.author | Shung, K. Kirk | - |
dc.date.available | 2017-07-11T06:19:05Z | - |
dc.date.created | 2017-04-10 | - |
dc.date.issued | 2014-09 | - |
dc.identifier.issn | 0301-5629 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/3048 | - |
dc.description.abstract | We describe how contactless high-frequency ultrasound microbeam stimulation (HFUMS) is capable of eliciting cytoplasmic calcium (Ca2+) elevation in human umbilical vein endothelial cells. The cellular mechanotransduction process, which includes cell sensing and adaptation to the mechanical micro-environment, has been studied extensively in recent years. A variety of tools for mechanical stimulation have been developed to produce cellular responses. We developed a novel tool, a highly focused ultrasound microbeam, for non-contact cell stimulation at a microscale. This tool, at 200 MHz, was applied to human umbilical vein endothelial cells to investigate its potential to elicit an elevation in cytoplasmic Ca2+ levels. It was found that the response was dose dependent, and moreover, extracellular Ca2+ and cytoplasmic Ca2+ stores were involved in the Ca2+ elevation. These results suggest that high-frequency ultrasound microbeam stimulation is potentially a novel non-contact tool for studying cellular mechanotransduction if the acoustic pressures at such high frequencies can be quantified. © 2014 World Federation for Ultrasound in Medicine & Biology. | - |
dc.publisher | Elsevier | - |
dc.title | NON-CONTACT HIGH-FREQUENCY ULTRASOUND MICROBEAM STIMULATION FOR STUDYING MECHANOTRANSDUCTION IN HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.ultrasmedbio.2014.03.018 | - |
dc.identifier.scopusid | 2-s2.0-84905573056 | - |
dc.identifier.bibliographicCitation | Ultrasound in Medicine and Biology, v.40, no.9, pp.2172 - 2182 | - |
dc.subject.keywordAuthor | High-frequency ultrasound microbeam | - |
dc.subject.keywordAuthor | Mechanotransduction | - |
dc.subject.keywordAuthor | Human umbilical vein endothelial cells | - |
dc.subject.keywordAuthor | Calcium fluorescence imaging | - |
dc.subject.keywordPlus | FLUID SHEAR-STRESS | - |
dc.subject.keywordPlus | CHONDROCYTES | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | HETEROGENEITY | - |
dc.subject.keywordPlus | TRANSDUCERS | - |
dc.subject.keywordPlus | MORPHOLOGY | - |
dc.subject.keywordPlus | INTENSITY | - |
dc.subject.keywordPlus | GRADIENTS | - |
dc.subject.keywordPlus | VIABILITY | - |
dc.subject.keywordPlus | MATRICES | - |
dc.citation.endPage | 2182 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 2172 | - |
dc.citation.title | Ultrasound in Medicine and Biology | - |
dc.citation.volume | 40 | - |
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