Cited 3 time in webofscience Cited 3 time in scopus

Phosphokinase Antibody Arrays on Dendron-Coated Surface

Title
Phosphokinase Antibody Arrays on Dendron-Coated Surface
Authors
Kwak, JW[Kwak, Ju-Won]Jeong, H[Jeong, Hyobin]Han, SH[Han, Sun-Ho]Kim, Y[Kim, Youngkyu]Son, SM[Son, Sung Min]Mook-Jung, I[Mook-Jung, Inhee]Hwang, D[Hwang, Daehee]Park, JW[Park, Joon Won]
DGIST Authors
Hwang, D[Hwang, Daehee]
Issue Date
2014-05-06
Citation
PLoS ONE, 9(5)
Type
Article
Article Type
Article
Keywords
AgeAlzheimer&aposs Disease (AD)Analytic MethodAnimalAnimal ExperimentAnimal ModelAnimal TissueAnimalsAnthracene DerivativeAnthracenesAntibodies, ImmobilizedAntigen-Antibody ReactionAntigen-Antibody ReactionsBinding KineticsBrainChemistryControlled StudyDendrimerDendrimersDendronDendron Coated Phosphokinase Antibody ArrayDisease ModelDisease Models, AnimalHumanHumansImmobilized AntibodyImmunoassayImmunologyMaleMiceMice, TransgenicMouseNon-HumanPathologyPhosphorylationPhosphotransferasePhosphotransferasesProceduresProtein PhosphorylationReproducibilitySensitivity and SpecificitySignal TransductionStereospecificityTransgenic Mouse
ISSN
1932-6203
Abstract
Monitoring protein phosphorylation at the cellular level is important to understand the intracellular signaling. Among the phosphoproteomics methods, phosphokinase antibody arrays have emerged as preferred tools to measure well-characterized phosphorylation in the intracellular signaling. Here, we present a dendron-coated phosphokinase antibody array (DPA) in which the antibodies are immobilized on a dendron-coated glass slide. Self-assembly of conically shaped dendrons well-controlled in size and structure resulted in precisely controlled lateral spacing between the immobilized phosphosite-specific antibodies, leading to minimized steric hindrance and improved antigen-antibody binding kinetics. These features increased sensitivity, selectivity, and reproducibility in measured amounts of protein phosphorylation. To demonstrate the utility of the DPA, we generated the phosphorylation profiles of brain tissue samples obtained from Alzheimer's disease (AD) model mice. The analysis of the profiles revealed signaling pathways deregulated during the course of AD progression. © 2014 Kwak et al.
URI
http://hdl.handle.net/20.500.11750/3088
DOI
10.1371/journal.pone.0096456
Publisher
Public Library of Science
Related Researcher
  • Author Hwang, Dae Hee Systems Biology and Medicine Lab
  • Research Interests Multilayered spatiotemporal networks; Regulatory motifs or pathways; Metabolite-protein networks; Network stochasticity; Proteomics and informatics
Files:
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Collection:
New BiologyETC1. Journal Articles


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