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Rhoeo discolor leaf extract as a novel immobilizing matrix for the fabrication of an electrochemical glucose and hydrogen peroxide biosensor

Title
Rhoeo discolor leaf extract as a novel immobilizing matrix for the fabrication of an electrochemical glucose and hydrogen peroxide biosensor
Authors
Nandini, S[Nandini, Seetharamaiah]Nalini, S[Nalini, Seetharamaiah]Shanmugam, S[Shanmugam, Sangaraju]Niranjana, P[Niranjana, Pathappa]Melo, JS[Melo, Jose Savio]Suresh, GS[Suresh, Gurukar Shivappa]
DGIST Authors
Shanmugam, S[Shanmugam, Sangaraju]
Issue Date
2014
Citation
Analytical Methods, 6(3), 863-877
Type
Article
Article Type
Article
Keywords
ChronoamperometryCyclic VoltammetryDifferential Pulse VoltammetryElectro-Chemical ElectrodesElectrocatalytic ActivityElectrochemical BiosensorElectrochemical PerformanceEnzyme ElectrodesFunctionalized Multi-Walled Carbon NanotubesGlucoseGlucose Oxidases (GOx)Glucose SensorsHorse-Radish PeroxidaseHydrogen PeroxideHydrogen Peroxide BiosensorsMatrix AlgebraMultiwalled Carbon Nanotubes (MWCN)Peroxides
ISSN
1759-9660
Abstract
A novel natural immobilizing matrix for the immobilization of glucose oxidase (GOx) and horseradish peroxidase (HRP) is presented in this article. The electrochemical biosensor was constructed by immobilizing the enzymes on Rhoeo discolor (Rd) leaf extract with 2.5% glutaraldehyde (GLD) on functionalized multiwalled carbon nanotubes (f-MWCNTs) modified graphite (Gr) electrode. The Gr/f-MWCNTs/(Rd-GLD)/GOx and Gr/f-MWCNTs/(Rd-GLD)/HRP biosensors showed excellent electrocatalytic activity concerning the detection of glucose and hydrogen peroxide. The physical morphology of the biosensors was studied using SEM and EDX. The electrochemical performance of the proposed biosensors was evaluated using cyclic voltammetry, differential pulse voltammetry and chronoamperometry. The effects of experimental variables such as pH, temperature, and applied potential on the current response of the biosensors were studied and optimized. The Gr/f-MWCNTs/(Rd-GLD)/GOx biosensor exhibited a rapid response time of less than 5 s, displayed a wide linear range of 0.5 to 28.5 mM, showed a low detection limit of 0.16 μM and revealed a high sensitivity of 15 μA mM-1 cm-2 for glucose. Similarly the Gr/f-MWCNTs/(Rd-GLD)/HRP biosensor showed a fast response time of 3 s, a good linear range of 0.2 to 6.8 mM with a 0.01 μM detection limit and an exceptional sensitivity of 2.1 mA mM-1 cm-2 for hydrogen peroxide. Subsequently, the practical applicability of the glucose biosensor for the analysis of glucose in Eleusine coracana wine and tender coconut water was examined while the Gr/f-MWCNTs/(Rd-GLD)/HRP modified electrode was tested for the determination of H2O2 in herbal bleach. In addition, the biosensors displayed long term stability, anti-interference ability and good reproducibility. © 2014 The Royal Society of Chemistry.
URI
http://hdl.handle.net/20.500.11750/3162
DOI
10.1039/c3ay41795f
Publisher
Royal Society of Chemistry
Related Researcher
Files:
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Collection:
Energy Science and EngineeringAdvanced Energy Materials Laboratory1. Journal Articles


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