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Nrg1 functions as a global transcriptional repressor of glucose-repressed genes through its direct binding to the specific promoter regions
- Nrg1 functions as a global transcriptional repressor of glucose-repressed genes through its direct binding to the specific promoter regions
- Lee, SB[Lee, Sung Bae]; Kang, HS[Kang, Hyen Sam]; Kim, T[Kim, TaeSoo]
- DGIST Authors
- Lee, SB[Lee, Sung Bae]
- Issue Date
- Biochemical and Biophysical Research Communications, 439(4), 501-505
- Article Type
- Beta-Fructofuranosidase; Binding Sites; Controlled Study; DNA Fragment; Galactokinase; Genes, Fungal; Glucose; Glucose Repression; HXT2; In Vivo Study; Non-Human; Nrg1; Nrg1 Protein; Priority Journal; Promoter Region; Promoter Regions, Genetic; Protein Binding; Protein Function; Repressor Proteins; Saccharomyces Cerevisiae; Saccharomyces Cerevisiae Proteins; SUC2; Transcription Factor; Transcription Factor HXT2; Transcription Factor SUC2; Transcription Regulation; Transcription, Genetic; Transcriptional Regulation; Unclassified Drug; Zinc Finger Protein
- Nrg1 is a zinc finger protein involved in the glucose repression of several glucose-repressed genes such as STA1, SUC2, and GAL1. Although the molecular details of the Nrg1-mediated repression of STA1 have been partly characterized, it still remains largely unknown how Nrg1 regulates these multiple target genes. In this study, we show that Nrg1 mediates the glucose repression of SUC2 and HXT2 through its direct binding to the specific promoter regions; it binds to the -404 to -360 region of the SUC2 promoter and the -957 to -810 region of the HXT2 promoter. Nrg1 also interacts with the -380 to -250 region of the PCK1 promoter, suggesting that it might also contribute to the PCK1 repression. In addition, ChIP assays confirmed that Nrg1 associated with specific promoter regions of these glucose-repressed genes in vivo. Analysis of the DNA fragments to which it binds indicates that Nrg1 may recognize T/ACCCC sequence within the promoters of these glucose-repressed genes as well as in its own promoter. Collectively, our findings indicate that Nrg1 mediates the glucose repression of multiple genes through its direct binding to the specific promoter regions. © 2013 Elsevier Inc.
- Academic Press Inc.
- Related Researcher
Lee, Sung Bae
SB LAB(Lab of Neurodegenerative diseases and Aging)
Cellular mechanism of neurodegenerative diseases; Neuronal maintenance and remodeling; 퇴행성 뇌질환의 세포기전; 신경계 유지 및 리모델링 연구
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