Cited 5 time in webofscience Cited 3 time in scopus

Nrg1 functions as a global transcriptional repressor of glucose-repressed genes through its direct binding to the specific promoter regions

Title
Nrg1 functions as a global transcriptional repressor of glucose-repressed genes through its direct binding to the specific promoter regions
Authors
Lee, SB[Lee, Sung Bae]Kang, HS[Kang, Hyen Sam]Kim, T[Kim, TaeSoo]
DGIST Authors
Lee, SB[Lee, Sung Bae]
Issue Date
2013-10-04
Citation
Biochemical and Biophysical Research Communications, 439(4), 501-505
Type
Article
Article Type
Article
Keywords
Beta-FructofuranosidaseBinding SitesControlled StudyDNA FragmentGalactokinaseGenes, FungalGlucoseGlucose RepressionHXT2In Vivo StudyNon-HumanNrg1Nrg1 ProteinPriority JournalPromoter RegionPromoter Regions, GeneticProtein BindingProtein FunctionRepressor ProteinsSaccharomyces CerevisiaeSaccharomyces Cerevisiae ProteinsSUC2Transcription FactorTranscription Factor HXT2Transcription Factor SUC2Transcription RegulationTranscription, GeneticTranscriptional RegulationUnclassified DrugZinc Finger Protein
ISSN
0006-291X
Abstract
Nrg1 is a zinc finger protein involved in the glucose repression of several glucose-repressed genes such as STA1, SUC2, and GAL1. Although the molecular details of the Nrg1-mediated repression of STA1 have been partly characterized, it still remains largely unknown how Nrg1 regulates these multiple target genes. In this study, we show that Nrg1 mediates the glucose repression of SUC2 and HXT2 through its direct binding to the specific promoter regions; it binds to the -404 to -360 region of the SUC2 promoter and the -957 to -810 region of the HXT2 promoter. Nrg1 also interacts with the -380 to -250 region of the PCK1 promoter, suggesting that it might also contribute to the PCK1 repression. In addition, ChIP assays confirmed that Nrg1 associated with specific promoter regions of these glucose-repressed genes in vivo. Analysis of the DNA fragments to which it binds indicates that Nrg1 may recognize T/ACCCC sequence within the promoters of these glucose-repressed genes as well as in its own promoter. Collectively, our findings indicate that Nrg1 mediates the glucose repression of multiple genes through its direct binding to the specific promoter regions. © 2013 Elsevier Inc.
URI
http://hdl.handle.net/20.500.11750/3190
DOI
10.1016/j.bbrc.2013.09.015
Publisher
Academic Press Inc.
Related Researcher
  • Author Lee, Sung Bae SB LAB(Lab of Neurodegenerative diseases and Aging)
  • Research Interests Cellular mechanism of neurodegenerative diseases; Neuronal maintenance and remodeling; 퇴행성 뇌질환의 세포기전; 신경계 유지 및 리모델링 연구
Files:
There are no files associated with this item.
Collection:
Brain and Cognitive SciencesETC1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE