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Morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditions

Title
Morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditions
Authors
Jung, GS[Jung, Gwon-Soo]Lee, KM[Lee, Kyeong-Min]Park, JK[Park, Jin-Kyu]Choi, SK[Choi, Seong-Kyoon]Jeon, WB[Jeon, Won Bae]
DGIST Authors
Jung, GS[Jung, Gwon-Soo]; Lee, KM[Lee, Kyeong-Min]; Park, JK[Park, Jin-Kyu]; Choi, SK[Choi, Seong-Kyoon]; Jeon, WB[Jeon, Won Bae]
Issue Date
2013-05-31
Citation
BMB Reports, 46(5), 276-281
Type
Article
Article Type
Article
Keywords
Antineoplastic AgentAntineoplastic AgentsBlotting, WesternCell Adhesion MoleculesCell CultureCell DifferentiationCell ProliferationCell SurvivalCells, CulturedDrug EffectExtracellular MatrixHumanHumansMulticellular NeurospheroidsMulticellular SpheroidNeuriteNeuritesNeuroblastomaNeuroblastoma CellsNeuronal DifferentiationPolymerase Chain ReactionRetinoic AcidSpheroids, CellularTretinoinUltrastructureWestern Blotting
ISSN
1976-6696
Abstract
In this study, we aimed to compare the morphogenetic and neuronal characteristics between monolayer cells and spheroids. For this purpose, we established spheroid formation by growing SH-SY5Y cells on the hydrophobic surfaces of thermally-collapsed elastin-like polypeptide. After 4 days of culture, the relative proliferation of the cells within spheroids was approximately 92% of the values for monolayer cultures. As measured by quantitative assays for mRNA and protein expressions, the production of synaptophysin and neuronspecific enolase (NSE) as well as the contents of cell adhesion molecules (CAMs) and extracellular matrix (ECM) proteins are much higher in spheroids than in monolayer cells. Under the all-trans-retinoic acid (RA)-induced differentiation condition, spheroids extended neurites and further up-regulated the expression of synaptophysin, NSE, CAMs, and ECM proteins. Our data indicate that RA-differentiated SH-SY5Y neurospheroids are functionally matured neuronal architectures. © 2013 by the The Korean Society for Biochemistry and Molecular Biology.
URI
http://hdl.handle.net/20.500.11750/3235
DOI
10.5483/BMBRep.2013.46.5.196
Publisher
Korean Society for Biochemistry and Molecular Biology
Related Researcher
Files:
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Collection:
Division of Nano∙Energy Convergence Research1. Journal Articles


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