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Electrochemical biosensor for the selective determination of hydrogen peroxide based on the co-deposition of palladium, horseradish peroxidase on functionalized-graphene modified graphite electrode as composite

Title
Electrochemical biosensor for the selective determination of hydrogen peroxide based on the co-deposition of palladium, horseradish peroxidase on functionalized-graphene modified graphite electrode as composite
Authors
Nandini, S[Nandini, Seetharamaiah]Nalini, S[Nalini, Seetharamaiah]Manjunatha, R[Manjunatha, Revanasiddappa]Shanmugam, S[Shanmugam, Sangaraju]Melo, JS[Melo, Jose Savio]Suresh, GS[Suresh, Gurukar Shivappa]
DGIST Authors
Shanmugam, S[Shanmugam, Sangaraju]
Issue Date
2013-01-15
Citation
Journal of Electroanalytical Chemistry, 689, 233-242
Type
Article
Article Type
Article
Keywords
Activation EnergyAmperometric BiosensorsAmperometric ResponseAnalytical PerformanceBiosensorsCodepositionCurrent ResponseCyclic VoltammetryDifferential Pulse Voltammetry TechniquesElectric ConductivityElectrical ConductivityElectro-Chemical Impedance Spectroscopy (EIS)Electrochemical BehaviorsElectrochemical BiosensorElectrochemical ResponseElectrodepositionEnergy Dispersive Analysis of X-RaysEnzyme ElectrodesEnzyme Modified ElectrodesFunctionalizedFunctionalized-GrapheneGrapheneGraphite ElectrodesHigh AffinityHorse-Radish PeroxidaseHydrogen PeroxideInterference SuppressionLinear RangeMichaelis-MentenModified ElectrodesModified Graphite ElectrodesOver PotentialPalladiumPhysical MorphologyReal SamplesReproducibilitiesScan RatesScanning Electron MicroscopySelective DeterminationSynergistic EffectTemperature Dependence
ISSN
1572-6657
Abstract
A sensitive and noble amperometric biosensor was developed by the co-deposition of palladium and horseradish peroxidase (HRP) on functionalized graphene (f-graphene) modified graphite electrode. The physical morphology of the modified electrodes was characterized using scanning electron microscopy and energy dispersive analysis of X-ray. The electrochemical behavior of the enzyme modified electrode towards hydrogen peroxide (H2O2) detection was evaluated using cyclic voltammetry and differential pulse voltammetry techniques where as the electrical conductivity and capacitance was investigated by electrochemical impedance spectroscopy. The effects of scan rate, pH, and temperature dependence on the current response of the biosensor were analyzed for sensitive analytical performance. The modified electrode exhibits a fast amperometric response of <2 s, good linear range of 25 μM-3.5 mM, effective sensitivity of 92.82 μA mM-1 cm -2, activation energy of 9.84 kJ M-1 and extraordinary Michaelis-Menten's constant (KMapp) of 0.11 mM. This could be attributed to high affinity, bioactivity of HRP and also due to the synergistic effects of HRP, Pd and f-graphene on the developed biosensor. The KMapp value obtained in this study is lower than those of the electrodes reported earlier. The modified electrode also reports a remarkable decrease of over potential for the detection of H2O2 that decreases the interference of the other substances and retains the selectivity for H2O2. The biosensor showed better reproducibility and stability with a good electrochemical response indicating that it can be stored up to 4 weeks without loosing its activity. In addition the modified electrode was utilized for real sample analysis that displayed good satisfactory results. © 2012 Elsevier B.V. All rights reserved.
URI
http://hdl.handle.net/20.500.11750/3270
DOI
10.1016/j.jelechem.2012.11.004
Publisher
Elsevier
Related Researcher
Files:
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Collection:
Energy Science and EngineeringAdvanced Energy Materials Laboratory1. Journal Articles


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