Cited 4 time in webofscience Cited 5 time in scopus

Sensitivity Enhancement of a Miniaturized Surface Plasmon Resonance Sensor Using Combination of Self-Assembled Mono layer and Protein G

Title
Sensitivity Enhancement of a Miniaturized Surface Plasmon Resonance Sensor Using Combination of Self-Assembled Mono layer and Protein G
Authors
Sin, EJ[Sin, Eun Jung]Lim, JO[Lim, Jeong-Ok]Sohn, YS[Sohn, Young-Soo]
DGIST Authors
Sin, EJ[Sin, Eun Jung]
Issue Date
2012-08
Citation
Nanoscience and Nanotechnology Letters, 4(8), 821-826
Type
Article
Article Type
Article
Keywords
AntibodiesBovine Serum AlbuminsC-Reactive ProteinsCardiovascular EventChemical DetectionChemical TreatmentsCRPDetection CapabilityEarly DiagnosisFlow ChannelsImmunosensorImmunosensorsLinker MoleculesProtein GSAMSAM LayerSelf Assembled MonolayersSensitivity EnhancementsSensorsSPRSPR Sensor ChipSPR SensorsSPR SystemsSurface Plasmon ResonanceSurface Plasmon Resonance Sensor
ISSN
1941-4900
Abstract
The detection capabilities of surface plasmon resonance (SPR) sensor chips modified with protein G, a self-assembled monolayer (SAM), and a combination of protein G and a SAM were investigated and compared using a miniaturized SPR sensor system. The miniaturized SPR system had two flow channels, with one used as a reference. To immobilize the antibodies on the SPR sensor chip for the detection of C-reactive protein (CRP), different linker molecules were formed on the gold SPR sensor chip at the beginning of the experiment. The CRP has been reported as a biomarker of future cardiovascular events. The antibody of CRP and bovine serum albumin (BSA) were then sequentially injected. The responses to CRP at concentrations of 0.5, 1, 3, and 5 μg/ml were observed and the sensitivities of each system compared. The SPR sensor chips modified with the protein G on the SAM layer exhibited the highest sensitivity and a stable linearity. The results demonstrated the feasibility of using the proposed SPR immunosensors for detecting CRP for early diagnosis without any additional chemical treatments. Copyright © 2012 American Scientific Publishers.
URI
http://hdl.handle.net/20.500.11750/3344
DOI
10.1166/nnl.2012.1400
Publisher
American Scientific Publishers
Files:
There are no files associated with this item.
Collection:
Robotics EngineeringBio-Micro Robotics Lab1. Journal Articles


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