Cited 19 time in webofscience Cited 20 time in scopus

An ISFET biosensor for the monitoring of maltose-induced conformational changes in MBP

Title
An ISFET biosensor for the monitoring of maltose-induced conformational changes in MBP
Authors
Park, HJ[Park, Hye-Jung]Kim, SK[Kim, Sang Kyu]Park, K[Park, Kyoungsook]Lyu, HK[Lyu, Hong-Kun]Lee, CS[Lee, Chang-Soo]Chung, SJ[Chung, Sang J.]Yun, WS[Yun, Wan Soo]Kim, M[Kim, Moonil]Chung, BH[Chung, Bong Hyun]
DGIST Authors
Lyu, HK[Lyu, Hong-Kun]
Issue Date
2009-01-05
Citation
FEBS Letters, 583(1), 157-162
Type
Article
Article Type
Article
Keywords
Biological MonitoringBiosensing TechniquesBiosensorCarrier ProteinsConformational ChangeConformational TransitionIon Sensitive Field Effect TransistorISFETMaltoseMaltose Binding ProteinMBPPriority JournalProtein ConformationSemiconductorsSurface Charge
ISSN
0014-5793
Abstract
Here we describe an ion sensitive field effect transistor (ISFET) biosensor, which was designed to monitor directly the surface charge of structurally altered maltose binding protein (MBP) upon stimulation with maltose. This study is the first report of the application of a FET biosensor to the monitoring of conformationally changed proteins. Consequently, a significant drop in current on the basis of the charge-dependent capacitance measurement has been clearly observed in response to maltose, but not for the glucose control, thereby indicating that the substrate-specific conformational properties of the target protein could be successfully monitored using the ISFET. Collectively, our results clearly suggest that ISFET provide a high fidelity system for the detection of maltose-induced structural alterations in MBP. © 2008 Federation of European Biochemical Societies.
URI
http://hdl.handle.net/20.500.11750/3551
DOI
10.1016/j.febslet.2008.11.039
Publisher
Wiley Oxford office
Related Researcher
Files:
There are no files associated with this item.
Collection:
Intelligent Devices and Systems Research Group1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE