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Detection of C-reactive protein on a functional poly(thiophene) self-assembled monolayer using surface plasmon resonance

Title
Detection of C-reactive protein on a functional poly(thiophene) self-assembled monolayer using surface plasmon resonance
Authors
Kim, HC[Kim, Hyun-Chul]Lee, SK[Lee, Soo-Keun]Jeon, WB[Jeon, Won Bae]Lyu, HK[Lyu, Hong-Kun]Lee, SW[Lee, Seung Woo]Jeong, SW[Jeong, Sang Won]
DGIST Authors
Kim, HC[Kim, Hyun-Chul]Lee, SK[Lee, Soo-Keun]Jeon, WB[Jeon, Won Bae]Lyu, HK[Lyu, Hong-Kun]Jeong, SW[Jeong, Sang Won]
Issue Date
2008-09
Citation
Ultramicroscopy, 108(10), 1379-1383
Type
Article
Article Type
Article; Proceedings Paper
Keywords
AmineAntibodyBiosensing TechniquesBiosensorC-Reactive ProteinC Reactive ProteinC Reactive Protein AntibodyChloroformEster DerivativeEster GroupsEsterificationEstersFerric ChlorideGel Permeation ChromatographyGoldInfrared SpectroscopyMolecular BiologyN-HydroxysuccinimideN HydroxysuccinimideNuclear Magnetic ResonanceOrganic CompoundsOrganic PolymersPoly(3 (2 ((N Succinimidyl)Succinyloxy)Ethyl)Thiophene)Poly(Thiophene)PolymerPolymerizationPolymersProtein AnalysisProtein InteractionSelf-Assembled MonolayerSelf AssemblySurface Plasmon ResonanceThiopheneThiophene DerivativeThiophenesUnclassified Drug
ISSN
0304-3991
Abstract
The preparation of a new poly(thiophene) with pendant N-hydroxysuccinimide ester groups and its application to immobilization of biomolecules are reported. A thiophene derivative of N-hydroxysuccinimide ester was polymerized with FeCl3 in chloroform and the resulting poly(thiophene) was characterized by nuclear magnetic resonance (NMR), Fourier transform infrared (FT-IR), and gel permeation chromatography (GPC). This polymer reacts with amine-bearing molecules to yield new poly(thiophene) derivatives and the specific interactions at the side groups could be detected. Thus, a self-assembled monolayer (SAM) using the polymer was formed on a gold-coated quartz cell and anti-C-reactive protein (anti-CRP) was immobilized. The binding behavior of CRP on the surface was monitored by use of a surface plasmon resonance (SPR) sensor system. © 2008 Elsevier B.V. All rights reserved.
URI
http://hdl.handle.net/20.500.11750/3564
DOI
10.1016/j.ultramic.2008.04.052
Publisher
Elsevier B.V.
Related Researcher
Files:
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Collection:
Division of IoT∙Robotics Convergence Research1. Journal Articles
Companion Diagnostics and Medical Technology Research Group1. Journal Articles


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