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Preparation of a sensing membrane for C-reactive protein

Title
Preparation of a sensing membrane for C-reactive protein
Authors
Kim, H.-C.[Kim, Huyn Chul]Lee, S.-K.[Lee, Soo Keun]Sohn, Y.-S.[Sohn, Young Soo]Ryu, H.-K.[Ryu, Hong K.]Jeong, S.W.[Jeong, Sang Won]
DGIST Authors
Kim, H.-C.[Kim, Huyn Chul]Lee, S.-K.[Lee, Soo Keun]; Sohn, Y.-S.[Sohn, Young Soo]; Ryu, H.-K.[Ryu, Hong K.]Jeong, S.W.[Jeong, Sang Won]
Issue Date
2007
Citation
Macromolecular Symposia, 249-250, 71-75
Type
Article
Article Type
Conference Paper
Keywords
BiomoleculesC-Reactive ProteinDNAImmobilizationProteinsSelf-Assembled MonolayerSelf Assembled MonolayersSensing MembraneSensor SystemsSensorsSurface Plasmon ResonanceSynthesis (Chemical)
ISSN
1022-1360
Abstract
11,11′-dithiobis(undecanyl succinate) (DS11SACOOH) was synthesized to develop a new surface for immobilization of proteins or antibodies on a gold substrate. DS11SACOOH is a bifunctional linker that has a disulfide group to form SAM (self-assembled monolayer) on a gold substrate and two carboxylic moieties for reaction with biomolecules such as peptide, DNA, etc. After preparation of SAM on a gold-coated quartz cell with DS11SACOOH, the SAM surface was activated with NHS (N-hydroxysuccimide). The terminal NHS ester was reacted with anti-CRP (C-reactive protein). The binding behavior of CRP on the surface was monitored by use of SPR (surface plasmon resonance) sensor system. Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA.
URI
http://hdl.handle.net/20.500.11750/3590
DOI
10.1002/masy.200750312
Publisher
John Wiley & Sons Ltd.
Related Researcher
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Collection:
Intelligent Devices and Systems Research Group1. Journal Articles
School of Undergraduate Studies1. Journal Articles
Division of IoT∙Robotics Convergence Research1. Journal Articles


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